Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1991-04-11
1995-10-10
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530380, 530384, 530413, 530416, C07K 116, C07K 118, C07K 122
Patent
active
054571812
ABSTRACT:
The present invention relates to a method for preparing a high purity Factor IX concentrate. The starting material for the process is the supernatant fraction of a cryoprecipitated human plasma. A pre-purification step is performed by DEAE-Sephadex chromatography. The resulting Factor IX fraction has a specific activity of at least 0.5 IU/mg protein. The purification method of the invention comprises two successive chromatography separations. First, ion-exchange chromatography on DEAE-sepharose is conducted so that the Factor IX is eluted when the ionic force of the buffer is increased to 0.34-0.38M sodium chloride. Then, affinity chromatography is conducted on heparin-sepharose. The elution buffer is a citrate buffer at a pH of 7.4 adjusted with 0.45M sodium chloride and supplemented with arginine as a stabilizer for Factor IX activity. Lysine is added as a stabilizer before freeze-drying of the purified Factor IX.
REFERENCES:
patent: 4081431 (1978-03-01), Stephan et al.
patent: 4374763 (1983-02-01), Takagi et al.
patent: 4397841 (1983-08-01), Johnson et al.
patent: 4440679 (1984-04-01), Fernandes et al.
patent: 4447416 (1984-05-01), Menache-Aronson
patent: 4540573 (1985-09-01), Neurath et al.
patent: 4777043 (1988-10-01), Bennett et al.
Hashimoto et al (1985), Journal of Biochemistry, vol. 97, pp. 1347-1355.
Bajaj et al (1982), Chemical Abstracts, vol. 96, p. 361, No. 100322g.
Chemical Abstracts, vol. 108, p. 345, No. 164242e (1988).
Journal of Chromatography, vol. 363 (1986), pp. 101-103.
Chemical Abstracts, vol. 107, No. 23, Dec. 7, 1987, p. 259, No. 213990n.
Fujikawa et al. (1973) Biochemistry 12, 4938-4945.
Pharmacia Fine Chemicals AB Publications (1980), Ion Exchange Chromatography: Principles and Methods, Uppsala.
Burnouf Thierry
Michalski Catherine
Centre Regional de Transfusion Sanguine de Lille
Lau Kawai
Wax Robert A.
LandOfFree
Preparation of a high-purity human factor IX concentrate and oth does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Preparation of a high-purity human factor IX concentrate and oth, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preparation of a high-purity human factor IX concentrate and oth will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2311003