Preparation of a compressed membrane containing immobilized biol

Details Preparation of a compressed membrane containing immobilized biol Preparation of a compressed membrane containing immobilized biol

1991-12-31

1994-02-01

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435182, 436531, 436535, 530815, 530817, C12N 1108, C12N 1104, G01N 33545, G01N 33544

Patent

active

052831868

ABSTRACT:
A porous carrier containing an immobilized biologically active material is prepared in which a relatively large amount of an enzyme or an antibody has been immobilized in a manner that produces a low diffusion resistance toward reagents and products. The porous carrier is produced by fixing enzymes or antibodies within internal micropores of the carrier and mechanically compressing the carrier to a final thickness which is in the range of about 0.20 to 0.80 times the uncompressed carrier thickness. The compressed carrier may have a density about 1.25 to about 5.0 times the density of the carrier before compressing. Surprisingly, the compressed carrier exhibits less diffusion resistance to specific reagents and products than would an uncompressed carrier. A preferred porous carrier is a semi-permeable membrane made from synthetic polymers, such as polyvinylidine difluoride.

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