Preparation of a cell culture substrate coated with poly-D-lysin

Details Preparation of a cell culture substrate coated with poly-D-lysin Preparation of a cell culture substrate coated with poly-D-lysin

1997-09-30

1999-08-03

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435174, 435176, 435177, 435180, 435395, 435402, C12M 300, C12N 1100, C12N 1108, C12N 500

Patent

active

059324732

ABSTRACT:
A cell culture substrate is coated with a composition containing a cell adhesion promoter in a salt solution. A substrate such as plastic, glass or microporous fibers is coated with a composition containing about 5 .mu.g/ml to about 1000 .mu.g/ml of poly-D-lysine in an 0.005 M to about 0.5 M citrate or sulfate salt solution to provide about 50 .mu.l to about 500 .mu.l of the composition per cm.sup.2 of substrate, and the coated substrate is rinsed to remove extraneous materials and dried to obtain a coated substrate having increased shelf-life and/or stability. The coated substrate may be sterilized by rinsing with a sterilizing medium such as ethanol.

REFERENCES:
patent: 5741701 (1998-04-01), Smiderek et al.

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