Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-12-26
1993-03-30
Naff, David M.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352401, 4352403, 435243, C12N 500, C12N 100
Patent
active
051983575
DESCRIPTION:
BRIEF SUMMARY
The present invention relates to a blood platelet lysate obtained from plasma from animal whole blood, a method for preparing the blood platelet lysate and a cell culture medium containing the blood platelet lysate.
A problem in the slaughterhouse industry in an international perspective is, in alia, to find a continuous and profitable market for slaughter blood. In many countries, the blood is even discarded as waste material for lack of possibilities of processing or using the blood. In cases where the slaughter blood is taken care of, it is mainly used as an admixture to foodstuffs and animal feed. The hemoglobin part of the blood can be used for different blood products, such as black-pudding, and the plasma part can be used as a thickener, a protein-enriching agent etc. Yet, there are not always marketing possibilities for all slaughter blood, and it is therefore important to find new fields of application.
In biotechnology, a further problem is that along with the ever increasing culture of mammalian cells and other animal cells for production and research, one of the main components of the growth medium, foetal calf serum (FCS), has become a limiting factor. Foetal calf serum (FCS) is extracted from calf foetus from slaughtered cows. There are also other drawbacks: FCS is expensive, and the quality when culturing a special cell line may vary strongly from batch to batch. Large efforts have therefore been made to develop alternatives to FCS.
We have now surprisingly found that the blood platelet fraction in the plasma part in blood is an excellent agent for cell culture, and that it can wholly or partly replace FCS, especially when culturing hybridoma cells.
Many different attempts to find alternatives to FCS are known. In U.S. Pat. No. 4,350,687 the object is to produce a single, simple and well-defined component to promote the growth of tumor cells. The source is platelets from human blood which is outdated and has become unfit for clinical use. This patent specification also states that extracts from platelets can promote the growth of 3T3 cells, SV3T3 cells and mouse fibroblasts which have been transformed. Gospadorowitz and Moran (Ann. Rev. Biochem. 45 (1976) 540) pointed out that the mitogenic factor for 3T3 cells should be present in platelets.
Platelet extracts are also known to stimulate primary cultures from pigs ("Serum-free medium enhances growth and differentiation of cultured pig granulosa cells" Barano J L S; Hammond J M, Milton S, Endocrinology 116 (1), 1985, 51-58), and "skin explants" ("Support and stimulation of epidermal cell outgrowth from porcines skin explants by platelet factors". Hebda PA, Alstadt SP, Hileman WT, Eaglstein WH. Br J Dermatol 1986 Nov; 115(5):529-41).
According to the classical theory, the role of the platelets is to aggregate on the site of the injury, to form a plug and to empty their contents of growth factors and other mediators, so as to stimulate connective tissue to grow, migrate and heal the wound, and to stimulate vascularisation.
A further attempt is stated in European patent specification 0,104,831. Here the starting material is material that normally goes to waste in slaughter, viz. "tissue juices together with flowing blood, a semi-solid, rubbery mass". The efficiency of this product is again demonstrated on cells which can be expected to respond in accordance with the classical theory: WI-38 (human fibroblasts); and on cell line VerO from African monkey kidney (transformed cells).
These previous attempts have all concerned either transformed cells or cells on which platelets can act in accordance with the classical theory.
The idea that platelets can play a role other than indicated by the classical theory, was recently suggested (Page, C.P. TIPS 9 (1988) 66-71). However, nowhere in this paper is it indicated that platelets or their contents stimulate the growth and division of cells which have been obtained from the immunological system.
The present invention is based on the unexpected and surprising result that a platelet extract stimulates the
REFERENCES:
patent: 4350687 (1982-09-01), Lipton et al.
patent: 4404279 (1983-09-01), Ricotti et al.
patent: 4473552 (1984-09-01), Jost
patent: 4959308 (1990-09-01), Ogden
patent: 4987079 (1991-01-01), Cullor
patent: 5045467 (1991-09-01), Bertheussen
patent: 5045468 (1991-09-01), Darfler
Annual Review of Biochemistry, vol. 45, 1976, E. E. Snell: "Growth Factors in Mammalian Cell Culture", see in particular p. 540.
Dialog Information Services, File 55 BIOSIS, Dialog Accession No. 0015173922, BIOSIS No. 7909715, Barano, J. L. S. et al., "Serum-Free Medium Enhances Growth and Differentiation of Cultured Pig Granulosa Cells", & Endocorinology 116 (1), 1985, 51-58.
Dialog Information Services, File 351 WPIL, Dialog Accession No. 2971640, WPI Acc No.: 82-19622E/10, (Haematology Blood): "Thrombocyte Concentrate Prodn. for Use in Haemastais Includes Treating Blood or Platelet Rich Plasma with Tetracycline Hydrochloride to Reduce Clumping", & SU 833246, A, 810531, 8210 (Basic).
Chemical Abstracts, vol. 95, No. 19, Nov. 9, 1981, (Columbus, Ohio, U.S.), see p. 122, Abstract 162602m, P. D. Phillips et al.: "Growth Regulation of WI138 Cells in a Serum-Free Medium", & Exp. Cell Res. 1981, 134(2), 297-302 (Eng).
Tips, vol. 9, Feb. 1988, C. P. Page: "The Involvement of Platelets in Non-Thrombotic Processes" (Elsevier Publications, Cambridge), pp. 66-70.
Holmovist Olov
Westermark Bengt
Ellco Food AB
Naff David M.
Ware Deborah K.
LandOfFree
Preparation of a blood platelet lysate for use in a cell culture does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Preparation of a blood platelet lysate for use in a cell culture, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preparation of a blood platelet lysate for use in a cell culture will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1280575