Preparation and use of recombinant plasmids containing genes for

Details Preparation and use of recombinant plasmids containing genes for Preparation and use of recombinant plasmids containing genes for

1980-08-01

1983-03-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 435196, 435253, 435317, 435820, 435849, 435881, C12N 1500, C12N 120, C12N 100, C12N 916

Patent

active

043755143

ABSTRACT:
PhoA mutant E. coli SB44, prepared by mutation of E. coli HB101 with an N-nitroso compound, can be used to identify and isolate recombinant plasmids into which a phoA gene has been incorporated. These plasmids can be used to transform bacteria which can be cloned and incubated to provide alkaline phosphatase in high yield. Moreover, these plasmid vectors can be modified in various ways so that the N-terminal amino acid sequence of phoA is followed in reading phase by the DNA coding for some other protein. In turn, these new plasmids can be used to transform bacteria which can be cloned and incubated to produce fusion proteins comprising the desired other protein in high yield and outside of the cell membrane in the periplasmatic space.

REFERENCES:
patent: 4190495 (1980-02-01), Curtiss
patent: 4237224 (1980-12-01), Cohen et al.
Bhatti, "Distinctive Characteristics of the Alkaline Phosphatase of Serratia Marcescens", Chem. Abstracts, vol. 83, No. 13 (1975), p. 224, Abs. No. 110308c.
Morriss et al., "Pheotriorn Effects of Mutations Involved in the Regulation of Escherichia coli K-12 Alkaline Phosphatase", J. Bact., vol. 119, No. 2, (1974), pp. 583-592.
Bracha, et al., "Location of the Genes Controlling Alkaline Phosphatase on F'13 Episone of Escherichia coli", J. Bact., vol. 120, No. 2 (1974), pp. 970-973.
Goebel et al., "Class of Small Multicopy Plasmids Originating from the Mutant Antibiotic Resistance Factor" R1 drd-19B2, J. Bact., vol. 123, No. 2, (1975), pp. 658-665.
Mercereau-Puijalon et al., "Synthesis of an Ovalbumin-Like Protein by Escherichia coli K12 Harbouring a Recombinant Plasmid", Nature, vol. 275, (1978), pp. 505-510.
Crosa et al., "Construction and Characterization of New Cloning Vehicles", Gene, vol. 2, (1977), pp. 95-113.

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