Preparation and use of gene banks of synthetic human antibodies

Details Preparation and use of gene banks of synthetic human antibodies Preparation and use of gene banks of synthetic human antibodies

1994-12-02

1998-11-24

Eisenschenk, Frank C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 691, 4352523, 4353201, 435326, 435 711, 5303873, 536 2353, 536 2433, 536 234, C12N 1513, C07H 2104, C07K 1900

Patent

active

058404791

ABSTRACT:
The invention relates to the preparation and use of gene banks of synthetic human antibodies (huAb) or parts of antibodies which contain the antigen-binding domain. Starting from a huAb framework in a suitable vector, the hypervariable regions of the antibody cDNA are formed by almost "randomly" combined oligonucleotides. Relatively conserved amino acids in the hypervariable regions have here been taken account of in the choice of appropriate nucleotides during the oligonucleotide synthesis and the ratio of the nucleotides used is likewise chosen such that a nonsense codon is to be expected at most in every 89th position. Expression of this synthetic huAb cDNA in microbial expression systems, e.g. in E. coli in the vector pFMT which is described below, thus makes a synthetic huAb library with a comprehensive repertoire for screening using selected antigens available in vitro.

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