Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of detaching cells – digesting tissue or establishing...
Patent
1996-06-06
1999-07-06
Naff, David M.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of detaching cells, digesting tissue or establishing...
435 11, 435325, 435378, 435394, C12N 500
Patent
active
059197030
ABSTRACT:
A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method includes contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold preservative solution to the warm digest, agitating the warm digest/cold preservative solution at a temperature between about 0.degree. and 15.degree. C., to thereby further digest the partially digested pancreas included in the warm digest, to form cold digest and collecting liquid from the cold digest to form isolated islets. The cold preservative solution and a pancreatic islet preservative solution of the present invention include D-mannitol, K-lactobionate and a buffer.
REFERENCES:
patent: 4797213 (1989-01-01), Parisius et al.
patent: 4798824 (1989-01-01), Belzer et al.
patent: 4868121 (1989-09-01), Scharp et al.
patent: 4873230 (1989-10-01), Belzer et al.
patent: 4879283 (1989-11-01), Belzer et al.
patent: 5200398 (1993-04-01), Strasberg et al.
patent: 5322790 (1994-06-01), Scharp et al.
patent: 5328821 (1994-07-01), Fisher et al.
patent: 5328844 (1994-07-01), Moore
patent: 5405742 (1995-04-01), Taylor
patent: 5411883 (1995-05-01), Boss et al.
patent: 5514536 (1996-05-01), Taylor
Benhamou et al., "Human Islet Isolation in 104 Consecutive Cases," Transplantation, 57(12), 1804-1810 (1994).
Benhamou, (ORDERED Oct. 8, 1996) Hormone & Metab. Res., 27, 113-120 (1995).
V.D.A. Delfino et al., "A Comparison of Four Solutions for Cold Storage of Pancreatic Islets," Transplantation, 56(6) 1325-1330 (1993).
Gray et al. (ORDERED Oct. 8, 1996) Stain Technology, 62, 373-381 (1987).
Kenmochi et al., "Improved Yield and Survival of Porcine Islets with Newly Developed Lap-1 Solution," Transplantation Proc., 26(6), 4012 (1994).
Ricordi et al., "Automated Method for Isolation of Human Pancreatic Islets," Diabetes, 37, 413-420 (1988).
van der Burg et al., "Pancreatic Islet Isolation with UW Solution: A New Concept," Transplantation Proc., 22(4), 2050-2251 (1990).
van der Burg et al., "Comparison of Islet Isolation Techniques in Dogs: Over 90% Purified Islets Using UW Solution," Transplantation, 22(2), 795-796 (1990).
van der Burg et al., "Metabolic Control After Autotransplantation of Highly Purified Canine Pancreatic Islets Isolated in UW Solution," Transplantation, 23(1), 785-786 (1991).
Kenmochi Takashi
Mullen Yoko
Naff David M.
The Regents of the University of California
Ware Deborah K.
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