Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1994-06-20
1997-03-04
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
530350, 530417, C12P 2106, C07K 112, C07K 14745
Patent
active
056078412
ABSTRACT:
A method for preparing a macromolecular protein complex (MPC) from fibrinogen in human plasma by the steps of ammonium sulfate precipitation, dialysis and gel filtration is disclosed. The isolated MPC may be degraded by contacting with trypsin. The isolated MPC inhibited fibrinolysis induced with plasminogen but not with plasmin. Elimination of the MPC by means of chondroitin sulfate A restored normal fibrinolysis. An antibody to fibrin-binding peptides which are produced by trypsin degradation of MPC, was conjugated to plasmin. The anti-MPC peptide antibody/plasmin conjugate has the capacity to bind to MPC-rich thrombus and degrade it without activation of fibrin-bound plasminogen.
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Weber Jon P.
Wityshyn Michael G.
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