Preparation and crystallization of fraction I protein from plant

Details Preparation and crystallization of fraction I protein from plant Preparation and crystallization of fraction I protein from plant

1980-11-03

1982-06-08

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435816, C12N 988

Patent

active

043340245

ABSTRACT:
A single, simple, uniform protocol for the rapid large scale purification and crystallization of ribulose 1,5-bisphosphate carboxylase (RuBisCO) to a greater than 90% purity from a wide variety of plant species, involving the steps of grinding and homogenizing plant material, such as leaves, with a suitable, acqueous buffer solution. The solution is filtered and the residue discarded. While maintaining temperature and pH control, sufficient quantities of polyethylene glycol (PEG) are added while stirring to bring the final concentration of PEG in the range of 8 to 15 (weight/volume) percent. To enhance crystal formation, magnesium chloride can be added to the solution. The precipitated material is discarded and the pure RuBisCO crystals which separate out of the remaining supernatant are collected, washed, dried and stored.

REFERENCES:
patent: 2600903 (1952-06-01), Miller
patent: 3780183 (1973-12-01), Edwards et al.
patent: 3823128 (1974-07-01), Bickoff et al.
patent: 4268632 (1981-05-01), Wildman et al.
Paulsen et al., Biochemistry, vol. 5, pp. 2350-2357, Jul. 1966.
Chan et al., Science, vol. 176, pp. 1145-1146, (1972).
Johal et al., Science, vol. 204, pp. 75-77, (Apr. 1979).
Kung et al. in Methods of Enzymology, vol. 69, pp. 326-336, (1980).

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