Preparation and application of artificial anti-idiotypic...

Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Polymers from only ethylenic monomers or processes of...

Reexamination Certificate

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C264S108000, C264S219000, C264S220000, C264S221000, C264S225000, C264S226000, C264S227000, C264S330000, C264SDIG004, C264S330000, C264S331160, C264S331190, C424S078080, C424S078370, C526S238200, C435S183000, C435S188500, C530S387100, C530S388100, C530S388900, C530S389800

Reexamination Certificate

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06489418

ABSTRACT:

The present invention concerns the preparation and application of artificial anti-idiotypic antibodies obtained by molecular imprinting.
It is known from Nature that antibodies can in their turn give rise to anti-antibodies. Such anti-antibodies or anti-idiotypic antibodies have been investigated widely [1]. The combining site of an anti-idiotypic antibody may display structural features which may be the “internal image” of the original antigen (the previous antibody). Attempts have also been described lately of producing monoclonal anti-idiotypic antibodies being functional internal images of enzyme active sites leading in one case to the formation of a catalytic antibody with cholinesterase activity [2]. This was carried out by allowing an antibody raised against an enzyme, i.e. cholinesterase, to be injected resulting in the aforementioned anti-anti-bodies.
A more presently developed technique, that of molecular imprinting, is a major element for the here described invention [3, 4]. It is the name given to a process for preparing polymers that are selective for a particular compound (the print molecule). The technique involves: (1) prearranging the print molecule and the monomers and allowing complementary interactions (non-covalent or reversible covalent) to develop; (2) polymerising around the print molecule-monomer complex; and (3) removing the print molecule from the polymer by extraction (FIG.
1
). Polymerisation thus preserves the complementarity to the print molecule and the polymer will selectively adsorb the print molecule subsequently. The technique has also been referred to as “host-guest” polymerisation or template polymerisation.


REFERENCES:
patent: 4447374 (1984-05-01), Tanaka
patent: 5110833 (1992-05-01), Mosbach
“Insulin's Structure as a Modified and Monomeric Molecule”, Ru Chang Bi et al.,Bipolymers,vol. 23, pp. 391-395 (1984).
“Structure and Stability of Insulin Dissolved in 1-Octanol”, James Matsuura et al.,J. Am. Chem. Soc.,1993, vol. 115, pp. 1261-1264.

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