Prenatal sex determination of bovine cells using male-specific o

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 91, 536 26, 536 27, 536 28, 436501, 436 94, C12P 1934, C12Q 768, C07H 1512, C07H 1700

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050553933

ABSTRACT:
Compositions and methods are provided for determining the sex of Bovine embryos and fetuses employing male-specific oligonucleotides in nucleic acid probe hybridization assay methods to assay the genomic DNA of embryonic or fetal cells for the presence of male-specific sequences.

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patent: 4683202 (1987-07-01), Mullis
patent: 4769319 (1988-09-01), Ellis et al.
Bondioli et al., "The Use of Male-Specific Chromosomal DNA Fragments to Determine the Sex of Bovine Preimplantation Embryos", Therigenology, 31:1, 1989, pp. 95-104.
Ellis et al., "Sex Determination of Bovine Embryos Using Male-Specific DNA Probes", Theriogeonology, 29:1, 1988, p. 242.
Herr et al., "Accuracy of a rapid Y-Chromosome-Detecting Bovine Embryo Sexing Assay", personal communication.
Herr et al., "Field Implementation of a Rapid Y-Chromosome-Detecting Bovine Embryo Sexing Assay", personal communication.
Handyside et al., "Biopsy of Human Preimplantation Embryos and Sexing by DNA Amplification", The Lancet, Feb. 18, 1989, pp. 347-349.
Kirszenbaum et al., "PCR Sexing Bovine Embryos Using Y Chromosome Specific Sequences", Journal of Cellular Biochemistry Abstracts, Supplement 13E, 1989, WH200, p. 293.
Leonard et al., "Sexing Bovine Embryos Using Y Chromosome Specific DNA Probe", Theriogenology, 27:1, 1987, p. 248.
Li et al., "Amplification and Analysis of DNA Sequences in Single Human Sperm and Diploid Cells", Nature, 35:29, 1988, pp. 414-417.

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