Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1988-07-25
1991-06-11
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 30, 435 32, 435 39, 435173, 4352402, 43524022, 435242, 435243, C12Q 102, C12N 1300, C12N 500, C12N 300
Patent
active
050231723
ABSTRACT:
An in vitro method for determining clonogenicity of and measuring the effect of physical (such as radiation or heat) or chemical treatment on mammalian test cells, including normal and tumor test cells. The method comprises encasing the test cells in agglomerates of non-proliferating, but metabolically active, feeder cells which are capable of forming spheroids. The resulting mixed (hybrid) multicellular spheroids provide an in vivo-like environment for the entrapped test cells. Test cells are subjected to treatment either before or after incorporation into spheroids, and the effect of such treatment is then evaluated from the colony forming ability of spheroids containing test cells.
REFERENCES:
patent: 4225486 (1980-09-01), Suzuki
patent: 4379839 (1983-04-01), Spiegelman
patent: 4423145 (1983-12-01), Stampfer et al.
Djordjevic et al., "Clonogenicity of Mammaliam Cells in Hybrid Spheroids: a New Assay Method", (1989), pp. 1-16.
B. Djordjevic et al., "Potentiation of Radiation Effects in Hypoxic HeLa Cells", Radiology, 125, pp. 525-527 (1977).
Djordejevic et al., "Responses of Synchronous Populations of HeLa Cells to Ultraviolet Irradiation at Selected Stages of the Generation Cycle", Radiation Res., 32, pp. 327-346 (1967).
R. Durand et al., "Effects of Intercellular Contact on Repair of Radiation Damage", Exp. Cell Res., 71, pp. 75-80, (1972).
A. Hamburger et al., "Primary Bioassay of Human Tumor Stem Cells", Science, vol. 78, pp. 461-463 (1977).
K. Kal and G. Barendsen, "In Vitro Methods for Predicting Response", in Cancer Treatment: End Point Evaluation, pp. 321-338 (1983).
L. Peters et al., "Predictive Assays of Tumor Radiocurability", Cancer Treatment Symposia, 1, pp. 67-74 (1984).
N. Pourreau-Schneider et al., "Relationship Between Surviving Fractions Using the Colony Method, the LD50, and the Growth Delay After Irradiation of Human Melanoma Cells Grown as Multicellular Spheroids", Radiation Res., 85, pp. 321-332 (1981).
M. Sapozink et al., "Effect of Hypoxia on Synchronous HeLa Cells Irradiated with Fast Neutrons", Radiology, 110, pp. 691-698, (1974).
R. Sutherland et al., "Growth of Multicell Spheroids in Tissue Culture as a Model of Nodular Carcinomas", J. Mat'l Cancer Inst., 46, pp. 113-120 (1971).
R. Sutherland et al., "A Multi-Component Radiation Survival Curve Using an In Vitro Tumor Model", Inst. J. Radiat. Biol., 18, pp. 491-495 (1970).
D. Von Hoff and L. Weisenthal, "In Vitro Methods to Predict for Patient Response to Chemotherapy", Adv. Pharmacol, Chemother., 17, pp. 133-156 (1980).
J. Yuhas et al., "A Simplified Method for Production and Growth of Multicellular Tumor Spheroids", Cancer Res., 37, pp. 3639-3642 (1977).
B. Djordjevic et al., "Oxygen Enhancement Ratios in HeLa Cells, Irradiated with Californium and Radium Sources", Radiology, 107, pp. 429-434 (1973).
B. Djordjevic, "Differential Effect of Procaine on Irradiated Mamalian Cells in Culture", Radiology, 131, pp. 515-519 (1979).
B. Djordjevic, "Modification of Radiation Response in HeLa Cells by Misonidazole", Int. J. Radiat. Biol., 36, pp. 601-612 (1979).
B. Djordjevic, "Variable Interaction of Heat and Procane in Potentiation of Radiation Lethality in Mammalian Cells of Neoplastic Origin", Int. J. Radiat. Biol., vol. 43, No. 4, pp. 399-409 (1983).
B. Djordjevic and J. Kim, "Modification of Radiation Response in Synchronized HeLa Cells by Metabolic Inhibitors: Effects of Inhibitors of DNA and Protein Synthesis", Radiat. Res., 37, pp. 435-450 (1969).
J. Hendry et al., "The Gastrointestinal Syndrome and Mucosal Clonogenic Cells: Relationships Between Target Cell Sensitivities, LD.sub.50 and Cell Survival, and Their Modification by Antiobiotics", Radiation Res., 96, pp. 100-112 (1983).
S. Kim et al., "Enhancement of the Radiation Response of Cultured Tumor Cells by Chloroquine", Cancer, 32, pp. 536-540 (1973).
C. Lange, "Studies on the Cellular Basis of Radiation Lethality: I. T
The invention described herein was supported by a grant awarded by the National Institutes of Health No. RO1 CA 35289.
Hoffer Florina B.
Kepplinger Esther L.
The Research Foundation of State University of New York
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