Precursors for two-step polynucleotide synthesis

Details Precursors for two-step polynucleotide synthesis Precursors for two-step polynucleotide synthesis

2003-08-30

2008-09-23

Crane, L. E. (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S026800, C536S027600, C536S027810, C536S028500

Reexamination Certificate

active

07427679

ABSTRACT:
Precursors for use in the synthesis of polynucleotides are disclosed. The precursors include a heterocyclic base having an exocyclic amine group and a substituted or unsubstituted triaryl methyl protecting group bound to the exocyclic amine group. In particular embodiments, the precursor has the structure:wherein:O and H represent oxygen and hydrogen, respectively,R1is hydrido, hydroxyl, protected hydroxyl, lower alkyl, modified lower alkyl, or alkoxy,one of R2or R3is a hydroxyl protecting group; and the other of R2or R3is a reactive group capable of reacting with a reactive site hydroxyl,Base is a heterocyclic base having an exocyclic amine group, andTram is a triaryl methyl group having the structure (V)wherein the broken line represents a bond to the amino nitrogen of the exocyclic amine group, and R4, R5and R6are independently selected from unsubstituted or substituted aryl groups, provided that at least one of R4, R5, and R6is an aryl group other than phenyl and other than substituted phenyl.

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