Precision determination for molecular weights

Details Precision determination for molecular weights Precision determination for molecular weights

1994-10-20

1996-06-18

Rosenberger, Richard A.

Optics: measuring and testing

By electrophoresis

G01N 2117

Patent

active

055283667

ABSTRACT:
This invention describes a method by which the precision of the reported molecular weights and radii of chromatographically separated molecules may be estimated. By suitably analyzing both the baseline and peak regions of a chromatogram, one may determine standard deviations of the signals from the various chromatographic detectors used in the analysis. This procedure yields standard deviations of the calculated values of molecular weight and root mean square radius of the separated molecules, as well as standard deviations of the calculated moments of the distribution of molecules. These standard deviations provide an estimate of the precision of the measurements.

REFERENCES:
patent: 4875169 (1989-10-01), Synovec et al.
Papazian et al. "Long and Short Range Precision in HPSEC," Journal of Liquid Chromatography, 13(1), 25-49 (1990).

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