Precision and accuracy of anion-exchange separation of nucleic a

Details Precision and accuracy of anion-exchange separation of nucleic a Precision and accuracy of anion-exchange separation of nucleic a

1995-08-16

1999-02-02

Ludlow, Jan

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

210656, 210660, 436161, 536 254, 935 19, G01N 3002

Patent

active

058664297

ABSTRACT:
Solvents for salt-gradient anion-exchange separation of nucleic acids, especially double-stranded DNA and especially by liquid chromatography, are improved by replacing NaCl as the eluting salt with any of a wide range of alkyl ammonium salts and can be used to elute nucleic acids in strict order of increasing length, with reduced sensitivity to elution temperature and salt concentration. Anion-exchange chromatography with these solvents is well suited for identification of DNA fragments on the basis of size, with greater accuracy, precision, and resolvable size range than often is possible with gel electrophoresis.

REFERENCES:
patent: 4039382 (1977-08-01), Thang et al.
Chemical Abstracts, CA 91:86673, Dizdaroglu et al., "Separation of small DNA and RNA oligonucleotides by high performance anion-exchange liquid chromatography," J. Chromatogr. (1979), 171, pp. 321-330.
Hecker et al. J. Chromatography v. 326 (1985) pp. 251-261.

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