Precision and accuracy of anion-exchange separation of nucleic a

Details Precision and accuracy of anion-exchange separation of nucleic a Precision and accuracy of anion-exchange separation of nucleic a

1996-01-23

1999-01-05

Ludlow, Jan

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preservative, buffer, anticoagulant or diluent

435 6, 436161, 536 254, 536 2643, 564281, 935 19, G01N 3002

Patent

active

058561927

ABSTRACT:
Solvents for salt-gradient anion-exchange separation of nucleic acids, especially double-stranded DNA and especially by liquid chromatography, are improved by replacing NaCl as the eluting salt with any of a wide range of alkyl ammonium salts and can be used to elute nucleic acids in strict order of increasing length, with reduced sensitivity to elution temperature and salt concentration. Anion-exchange chromatography with these solvents is well suited for identification of DNA fragments on the basis of size, with greater accuracy, precision, and resolvable size range than often is possible with gel electrophoresis.

REFERENCES:
patent: 4039382 (1977-08-01), Thang et al.
patent: 4110360 (1978-08-01), Sheldon et al.
patent: 4115216 (1978-09-01), Skaletz
patent: 4136175 (1979-01-01), Rideout et al.
patent: 4560447 (1985-12-01), Shono
patent: 4711955 (1987-12-01), Ward et al.
patent: 4917781 (1990-04-01), Sharifian et al.
patent: 4935342 (1990-06-01), Seligson et al.
patent: 4950368 (1990-08-01), Weinberg et al.
patent: 4965199 (1990-10-01), Capon et al.
patent: 5010183 (1991-04-01), Macfarlane
patent: 5087559 (1992-02-01), Smith et al.
patent: 5118672 (1992-06-01), Schinazi et al.
patent: 5175269 (1992-12-01), Stavrianopoulos
patent: 5702896 (1997-12-01), Collins et al.
Spittler et al., J. Chromatogr. vol. 317 pp. 527-531, 1984.
Robinson and Grant, 1966, J. Biol. Chem. 241:4030-4042.
Shapiro et al, 1969, Biochemistry 8:3219-3232.
Shapiro et al, 1969, Biochemistry 8:3233-3241.
Melchior and von Hippel, 1973, Proc. Natl. Acad. Sci. USA 70:298-302.
Orosz and Wetmur, 1977, Biopolymers 16:1183-1199.
Shore et al, 1981, Proc. Natl. Acad. Sci. USA 78:4833-4837.
Kato et al, 1983, J. Chromatography 265:342-346.
Hagerman, 1985, Biochemistry 24:7033-7037.
Hecker et al, 1985, J. Chromatography 326:251-261.
Eriksson et al, 1986, J. Chromatography 359:265-274.
Muller, 1986, European Journal of Biochemistry 155:203-212.
Thompson, 1986, BioChromatography 1(1):16-20.
Zon and Thompson, 1986, BioChromatography 1(1)22-32.
Thompson, 1986, BioChromatography 1(2):68-80.
Thompson, 1987, BioChromatography 2(1):4-18.
Westman et al, 1987, Anal. Biochem. 166:158-171.
Koo and Crothers, 1988, Proc. Natl. Acad. Sci. USA 85:1763-1767.
Merion et al, 1988, BioTechniques 6:246-251.
Kato et al, 1989, J. Chromatography 478:264-268.
Hagerman, 1990, Annual Reviews of Biochemistry 59:755-781.
Maa et al, 1990, J. Chromatography 508:61-73.
Journal of Chromatography, vol. 467(1990), No. 1, pp. 217-226, Ellegren et al, Size-Exclusion Chromatography of DNA Restriction Fragments -- Fragment Length Determinations and a Comparison with the Behavior of Proteins Size-Exclusion Chromatography.
Chemical Reviews, vol. 89(1989), pp. 309-319, Larry McLaughlin, "Mixed-Mode Chromatography of Nucleic Acids".
Journal of Chromatography, vol. 533(1990), pp. 87-96, R.G. Brownlee et al, "Application of Capillary DNA Chromatography to Detect AIDS Virus (HIV-1) DNA in Blood".
Journal of Chromatography, vol. 359(1986), pp. 265-274, S. Eriksson et al, "Separation of DNA Restriction Fragments by Ion-Pair Chromatography".
Nucleic Acids Research, vol. 19(1991), pp. 4181-4188, F. Fach et al, "DNA Fragments Display Retarded Elution upon Anion-Exchange HPLC".

Affiliated with

Also associated with

Rating

Precision and accuracy of anion-exchange separation of nucleic a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Precision and accuracy of anion-exchange separation of nucleic a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Precision and accuracy of anion-exchange separation of nucleic a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-861648