Precipitation of interfering proteins in fluorescence polarizati

Chemistry: analytical and immunological testing – Including sample preparation – Digestion or removing interfering materials

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436546, 436817, 436825, G01N 100, G01N 33533

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active

047343788

ABSTRACT:
Interfering proteins are precipitated and digoxin extracted in a fluorescence polarization immunoassay for digoxin with a protein precipitating reagent. The reagent contains about 3 to 4% 5-sulfosalicyclic acid in an aqueous solution including about 40 to 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.

REFERENCES:
patent: 4420568 (1983-12-01), Wang et al.
patent: 4430414 (1984-02-01), Swanson
patent: 4654311 (1987-03-01), Khanna et al.
patent: 4698315 (1987-10-01), Farrenkopf
Agric. Biol. Chem., 43 (7), 1473-1478, 1979, Ohara et al.
Clin. Chem. 30/11, 1826-1829 (1984), Porter et al.
Russian Article, Dissociation Constants of Sulfosalicylic Acid in Mixed Solvents, Porfir'eva et al.

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