Chemistry: physical processes – Physical processes – Crystallization
Patent
1978-12-04
1980-08-05
Turk, Arnold
Chemistry: physical processes
Physical processes
Crystallization
252408, G01N 3316
Patent
active
042159938
ABSTRACT:
A precipitating reagent and method is provided for isolating high density lipoproteins from low density lipoproteins in human serum together with a quantitative determination of high density lipoprotein cholesterol. Precipitation of low density lipoproteins is accomplished by the precipitating reagent without the addition of metal ions into the sample. The precipitating reagent lowers the pH of the human serum approximately to the isoelectric point of the low density lipoproteins through the use of an organic buffer. The precipitating reagent also contains a polyanion and neutral polymer. The preferred composition of the precipitating reagent contains about 0.4% phosphotungstic acid by weight thereof, about 2.5% of polyethylene glycol by weight thereof and 2-(N-morpholino) ethane sulfonic acid as the buffer present in a concentration of from about 0.2 molar to about 0.5 molar. According to the method provided, the precipitating reagent is added to the human serum sample thereby causing the low density lipoproteins to form a precipitate, leaving the high density lipoproteins in the resulting supernatant liquid. The supernatant is separated from the precipitate and a cholesterol assay reagent is added to the supernatant. The cholesterol assay reagent reacts with the high density lipoprotein to produce a compound that absorbs radiation at a specific wavelength. The amount of high density lipoprotein cholesterol present in the human serum sample is then determined by comparing the absorbance of a sample with the absorbance of a known standard.
REFERENCES:
patent: 3770631 (1973-11-01), Fekete et al.
patent: 3955925 (1976-05-01), Proksch et al.
patent: 4086218 (1978-04-01), Shanbrom et al.
patent: 4110077 (1978-08-01), Klein et al.
patent: 4126416 (1978-11-01), Sears
Data Medical Associates, Inc.
Turk Arnold
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