Precipitate test for microorganisms

Details Precipitate test for microorganisms Precipitate test for microorganisms

1993-04-21

1995-04-04

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 38, 435288, 435296, 435805, 435970, 436533, 436534, C12Q 100, C12Q 108, C12Q 110

Patent

active

054037212

ABSTRACT:
There is disclosed an improved method for testing for the presence of a particular microorganism or group of microorganisms characterized by a particular enzyme. The method uses a dye-forming substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate when cleaved by the enzyme. The precipitate is concentrated to the polymer matrix and/or solid support member to create a visible reaction product, wherein the amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming substrate throughout a polymer system or on the surface of a solid support member.

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