Pre-treatment kit for saliva and pre-treatment method for...

Details Pre-treatment kit for saliva and pre-treatment method for... Pre-treatment kit for saliva and pre-treatment method for...

2005-05-24

2005-05-24

Leary, Louise N. (Department: 1654)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S975000, C435S885000

Reexamination Certificate

active

06897037

ABSTRACT:
To provide a pre-treatment kit for saliva and a pre-treatment method for saliva for identification and quantitation of mutans streptococci in human saliva by the immunochromatographic method, which can eliminate mucin in saliva and prevent mutans streptococci from chaining and aggregation in a simple method, the pre-treatment kit is constructed of (A) an aqueous solution containing sodium hydroxide, (B) a tris(hydroxymethyl)aminomethane buffer solution containing tartaric acid and/or citric acid, and (C) a nonionic surfactant and/or an amphoteric surfactant, wherein the component (C) is previously mixed with or prepared separately from the component (A) and/or the component (B), and further (D) a pH indicator having a color transition range of pH 5 to 9 is previously mixed with or prepared separately from the component (A) or the component (A) having the component (C) previously mixed therewith, and/or the component (B) or the component (B) having the component (C) previously mixed therewith. According the pre-treatment method, the respective components of the pre-treatment kit is added dropwise into and mixed with saliva in an arbitrary order.

REFERENCES:
patent: 20020197738 (2002-12-01), Matsumoto et al.
Courtney et al, “Infection and immunity”, V.59(5), p1661-1666, (May, 1991) (Abstract Only).*
Twetman et al, “Int. J Paediatric Denistry”, V.9(2), p 93-98, (Jun. 1999) (Abstract Only).

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