Pre-mRNA processing enhancer and method for intron-independent g

Chemistry: molecular biology and microbiology – Vector – per se

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

536 231, 536 241, C12N 1563

Patent

active

059142677

ABSTRACT:
A chimeric RNA molecule comprising at least one pre-mRNA processing element is disclosed. A gene construct comprising a DNA sequence encoding at least one pre-mRNA processing enhancer is also disclosed. A method of enhancing cytoplasmic RNA accumulation is disclosed. This method comprises the step of inserting a DNA sequence encoding the RNA into the vector described and expressing the DNA sequence.

REFERENCES:
patent: 4666848 (1987-05-01), Gelfand et al.
Buchman, A.R., et al., "Comparison of Intron-Dependent and Intron-Independent Gene Expression," Mol. Cell. Biol. 8(10):4395-4405, 1988.
Greenspan, D.S., et al., "Synthesis of Predominantly Unspliced Cytoplasmic RNAs by Chimeric Herpes Simplex Virus Type 1 Thymidine Kinase-Human .beta.-Globin Genes." Mol. Cell. Biol. 5(8)1894-1900, 1985.
Huang and Gorman, "Intervening Sequences Increase Efficiency of RNA 3' Processing and Accumulation of Cytoplasmic RNA," Nucl. Acids Res. 18:937-947, 1990.
Kashiwagi, et al., "Involvement of Ribonuclease III in the Enhancement of Expression of the speF-potE Operon," Biochem. Biophys. Res. Commun. 200:591-597, 1994.
Legrain, P., et al., "Some Cis-and Trans-Acting Mutants for splicing Target Pre-mRNA to the Cytoplasm," Cell 57:573-583, 1989.
Liu, X., et al., "A 68 kDa Sequence-specific RNA Binding Protein Involved in Processing of Intronless pre-mRNAs," p. 258, abstract presented at "RNA Processing Meeting" at RNA Society Meeting, May 28, 1994.
Liu, X., "Effects of Intron and Exon Sequence Elements on Intron-dependent and Intron-independent Gene Expression," Ph.D. thesis, catalogued on Nov. 24, 1994.
McKnight, S.L., "The Nucleotide Sequence and Transcript Map of the Herpes Simplex Virus Thymidine Kinase Gene," Nucl. Acids. res. 8(24):5949-5963, 1980.
Prescott and Falck-Pedersen, "Sequence Elements Upstream of the 3' Cleavage Site Confer Substrate Strength to the Adenovirus L1 and L3 polyadenylation sites," Mol. Cell. Biol. 14:4682-4693, 1994.
Sjorberg, et al., A significantly Improved Semliki Forest Virus Expression System Based on the Translation Enhancer Segments from the Viral Capsid Gene, Biotechnol. 12:1127-1131, 1994.
Wagner, et al., "Nucleotide Sequence of the Thymidine Kinase Gene," Proc. Natl. Acad. Sci. USA 78:1441-1445, 1981.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Pre-mRNA processing enhancer and method for intron-independent g does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Pre-mRNA processing enhancer and method for intron-independent g, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pre-mRNA processing enhancer and method for intron-independent g will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1707903

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.