Pre-cast gel systems for two-dimensional electrophoresis

Details Pre-cast gel systems for two-dimensional electrophoresis Pre-cast gel systems for two-dimensional electrophoresis

1988-11-04

1989-10-17

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041828, G01N 2726

Patent

active

048744905

ABSTRACT:
A strip gel and a slab gel are combined in a single gel enclosure or on a single gel backing for two-dimensional electrophoresis. The enclosure is comprised of a single pair of support plates, and a removable electrically insulating region is included to separate the gels. The region is occupied either by air or an electrically nonconductive material which can be removed without disturbing either gel. In use, a sample is loaded onto one end of the strip gel and an electric potential is imposed across the strip gel for the first dimension separation while the strip gel is electrically insulated from the slab gel. The insulating region is then removed and the two gels placed in electrical contact for the second dimension separation.

REFERENCES:
patent: 4385974 (1983-05-01), Shevitz
patent: 4443319 (1984-04-01), Chait et al.

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