Practical serological assay for the clinical diagnosis of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S005000, C435S006120, C435S007220, C424S269100, C424S093100, C530S350000

Reexamination Certificate

active

07008774

ABSTRACT:
Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoaLeishmaniais disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies toLeishmaniaparasite soluble antigens and antigen-capture ELISAs for the detection ofLeishmaniaparasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection ofLeishmaniaparasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization ofLeishmaniaamastigotes or promastigotes in a sample.

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