Poxvirus insertion/expression vector

Details Poxvirus insertion/expression vector Poxvirus insertion/expression vector

1987-08-10

1995-08-22

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 241, 4352351, 4353201, 4352402, 935 32, 935 34, 935 70, C12N 1586, C12N 1511, C12N 516, C12P 2100

Patent

active

054439647

ABSTRACT:
The invention comprises a plasmid vector, utilizing genetic material that causes high levels of expression of poxvirus genes, and the method of using this genetic material. One preferred embodiment comprises an upstream cis-acting element of a poxvirus gene encoding the major protein component of the poxvirus A-type inclusion. A second preferred embodiment of the invention comprises a downstream cis-acting element of a poxvirus gene encoding the major protein component of the poxvirus A-type inclusion. These elements are inserted upstream and downstream respectively, of selected cloned genes in the plasmid vector to obtain very high levels of expression of the cloned gene.

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