Potyvirus vectors for the expression of foreign genes

Details Potyvirus vectors for the expression of foreign genes Potyvirus vectors for the expression of foreign genes

1995-06-06

1998-06-16

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 691, 435 694, 435 6952, 435 696, 435 911, 435 9133, 435410, 435419, C12N 701, C12N 1501, C12P 2100

Patent

active

057668850

ABSTRACT:
The present invention provides an expression vector adapted for expressing heterologous proteins in plants susceptible to a polyprotein-producing plant virus. The vector utilizes the unique ability of viral polyprotein proteases to cleave heterologous proteins from viral polyproteins. Also provided is a method for expressing heterologous proteins in plants using these unique expression vectors.

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patent: 4874703 (1989-10-01), Jaskunas
patent: 5162601 (1992-11-01), Slightom
Dolja, V.V., et al., Proc. Natl. Acad. Sci., 89:10208-10212 (1992).
Dolja, V.V. et al., J. Virol., 67(10);5968-5875 (1993).
Donson, et al. Proc. Natl. Acad. Sci. USA 88:7204-7208 (1991).
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Carrington J.C. et al. J. Virol. 64(4):1590-1597 (1990).
R.E.F. Matthews (1991) In: Plant Virology , 3.sup.rd. ed., Academic Press, San Diego, CA, pp. 486-487.

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