Chemistry: electrical and wave energy – Processes and products
Patent
1978-08-28
1982-07-20
Kaplan, G. L.
Chemistry: electrical and wave energy
Processes and products
204195P, 204195B, 435 4, 435 14, 435291, 435817, C12Q 126, C12Q 130, G01N 2746
Patent
active
043404480
ABSTRACT:
A method for potentiometric detection of hydrogen peroxide including providing an electrolytic cell having a working electrode and a reference electrode. The working electrode is composed of a hydrogen peroxide sensitive material and also is employed as a support for an oxidase enzyme. Introducing a hydrogen peroxide releasing substance into the cell and releasing the hydrogen peroxide by effecting interaction with the enzyme. Developing in said cell an electrical potential which is a function of the concentration of the hydrogen peroxide and measuring the electrical potential of the cell. The method may be employed as a quantitative glucose detector by employing as the enzyme or enzymes glucose oxidase with or without catalase.
Apparatus for potentiometric determination of hydrogen peroxide includes a cell containing an electrolyte, a working electrode and a reference electrode. An electrometer is operatively associated with the electrodes. The working electrode is composed of a material which will create an electrical potential when it interacts with hydrogen peroxide. At least one oxidase enzyme is immobilized on the working electrode.
REFERENCES:
patent: 2850359 (1958-09-01), Worthington et al.
patent: 3403081 (1968-09-01), Rohrback et al.
patent: 3539455 (1970-11-01), Clark
patent: 3542662 (1970-11-01), Hicks et al.
patent: 3556945 (1971-01-01), Messing
patent: 3595755 (1971-07-01), Hartel
patent: 3666733 (1972-05-01), Epton
patent: 3707455 (1972-12-01), Derr et al.
patent: 3770607 (1973-11-01), Williams
patent: 3788950 (1974-01-01), Hicks et al.
patent: 3838011 (1974-09-01), Hagen
patent: 3838033 (1974-09-01), Mindt et al.
patent: 3839154 (1974-10-01), Messing
patent: 3841971 (1974-10-01), Messing
patent: 3857771 (1974-12-01), Sternberg
patent: 3920969 (1975-11-01), Berglas
patent: 3966580 (1976-06-01), Janata et al.
patent: 4024042 (1977-05-01), Enfors et al.
patent: 4055175 (1977-10-01), Clemens et al.
David A. Gough et al., Science, vol. 180, pp. 380-384, (1973).
J. N. Groves, General Electric Report No. 70-C-271, pp. 1-11, Nov. 1970.
S. P. Bessman et al., "Stabilized Glucose Oxidase Electrode for Monitoring Glucose in Biological Fluids", Methods and Instrumentation in Medical Engineering.
Liu Chung-Chiun
Schiller Julian G.
Wingard, Jr. Lemuel B.
Kaplan G. L.
University of Pittsburgh
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