Potent genetic switch allowing regulated gene expression in euka

Details Potent genetic switch allowing regulated gene expression in euka Potent genetic switch allowing regulated gene expression in euka

1998-08-03

1999-11-23

Degen, Nancy

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435352, 435455, 435465, 536 231, 536 234, C12N 500

Patent

active

059899107

ABSTRACT:
The present invention provides a system for controlled transgene transcription using chimeric activator and repressor proteins functioning in a novel regulatory network. The target transgene is actively silenced in non-inducing conditions by a novel class of chimeric proteins consisting of the DNA-binding tetracycline repressor fused to distinct multimerized eukaryotic transcriptional repression domains. In the presence of a tetracycline "inducer", the repressor does not bind to the promoters for both the target gene and for another regulatory gene encoding a transactivator (e.g., GAL4-VP16). Under these inducing conditions, the transactivator activates expression of the target transgene and of its own gene, in an additional autoregulatory positive feedback loop.

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