Potent antigen presenting cell composition

Details Potent antigen presenting cell composition Potent antigen presenting cell composition

1995-07-12

2000-09-19

Saunders, David

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435372, 435373, 435375, 435395, 435382, 435 2, C12N 500, C12N 502, C12N 506

Patent

active

061210447

ABSTRACT:
A method of obtaining a blood-cell fraction enriched for potent antigen presenting cells is disclosed. The method includes obtaining a monocyte-depleted lymphocyte fraction, culturing the cell fraction in a serum-free medium for a period sufficient to produce a morphological change in dendritic-precursor cells to cells having the morphology of dendritic cells, harvesting non-adherent cells produced by said culturing, and enriching the portion of dendritic cells in the harvested cells by density centrifugation. Also disclosed is a PAP cell composition containing cells enriched for PAP activity in a collagen matrix.

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