Potency and atoxicity test for modified neurotoxin

Details Potency and atoxicity test for modified neurotoxin Potency and atoxicity test for modified neurotoxin

1977-06-17

1979-07-24

Fagelson, Anna P.

Drug, bio-affecting and body treating compositions

In vivo diagnosis or in vivo testing

Magnetic imaging agent

424 89, 424 93, 424 98, 435235, 435236, 435237, 435240, 435241, A61K 2900, A61K 3558, C12K 900

Patent

active

041623037

ABSTRACT:
A method for proving the potency and atoxicity of modified neurotoxin is provided. A thin sheet of viable cells is provided on a growth substrate. Viability of the sheet of cells is defined as exhibiting an uninhibited growth potential of at least 10.sup.6 Plaque Forming Units of Semliki Forest virus. The sheet of cells is treated with the test modified neurotoxin and then inoculated with the Semliki Forest virus. The reduction in the number of plaques of Semliki Forest virus in the culture indicates the potency of the modified neurotoxin, since the modified neurotoxin interferes with plaque formation by the Semliki Forest virus in proportion to its potency. Atoxicity of the modified neurotoxin is demonstrated by lack of cell destruction.

REFERENCES:
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Frankel-Conrat (Ed) Comprehensive Virol., vol. 2, Cpt. 4, 1974, pp. 171-173, 176, 177, 187-189.
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Wyler, Chem. Abs., vol. 82, 1975, Ab. No. 165292c.
Miller, Biochim et Biophys. Acta, vol. 496, Jan. 24, 1977, pp. 192-196.

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