Potassium-dependent sodium-calcium exchanger

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C536S023500, C435S069100, C435S252300, C435S325000, C435S320100, C530S350000

Reexamination Certificate

active

10506624

ABSTRACT:
A novel polypeptide, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, a cell transfected by the expression vector, a method for producing the polypeptide, and a convenient screening system for obtaining a substance useful in treating cell injuries due to postischemic reperfusion or an inflammatory disease, are disclosed.The polypeptide is a potassium-dependent sodium-calcium exchanger expressed in peripheral leukocytes.

REFERENCES:
patent: 6787352 (2004-09-01), Friddle et al.
patent: 9-67336 (1997-03-01), None
patent: 97/09306 (1997-03-01), None
patent: WO 01/83512 (2001-11-01), None
patent: 02/26980 (2002-04-01), None
Balasubramanyam M et al. Na+/Ca2+ exchange-mediated calcium entry in human lymphocytes. J Clin Invest, 1994; 94: 2002-2008.
Harada N et al. Adenosine and selective A2A receptor agonists reduce ischemia/reperfusion injury of rat liver mainly by inhibiting leukocyte activation. J Pharm Exp Therap. 2000; 294(3): 1034-1042.
Mochizuki S and Jiang C. Na+/Ca2+ exchanger and myocardial ischemia/reperfusion. Japanese Heart J, 1998; 39(6): 707-714.
Siegl et al.; “Inhibition of Na+/Ca2+Exchange in Membrane Vesicle and Papillary Muscle Preparations From Guinea Pig Heart by Analogs of Amiloride”; Proc. Natl. Acad. Sci. USA, vol. 81, pp. 3238-3242, (1984).
International Preliminary Examination Report for application PCT/JP2003/009732 filed Jul. 31, 2003.
EPO Communication and Supplementary Partial European Search Report mailed Nov. 8, 2005, for application EP 03 76 6674.
Li et al., “Molecular Cloning of a Fourth Member of the Potassium-dependent Sodium-Calcium Exchanger Gene Family, NCKX4,” Journal of Biological Chemistry, 277(50):48410-48417 (2002).

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