Post-transcriptional heme regulated heterologous gene expression

Details Post-transcriptional heme regulated heterologous gene expression Post-transcriptional heme regulated heterologous gene expression

1986-06-13

1989-07-18

Teskin, Robin Lyn

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 4351723, 435255, 435256, 435320, 935 28, 935 37, 935 56, 935 69, 536 27, C12P 2100, C12N 1500, C12N 500, C07H 1512

Patent

active

048493481

ABSTRACT:
A method for controlling the level of heterologous gene expression in yeast using a soybean leghemoglobin leader sequence and a suitable promotor. Levels of expression are controlled at the post-transcriptional level by adjusting the amount of heme in the yeast culture medium.

REFERENCES:
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Hamilton et al, Proc. Natl. Acad. Sci., vol. 79, pp. 7609-7613, Dec. 1982, "Translational Control of Catalase Synthesis by Hemin in the Yeast Saccharomyces Cerevisiae".
Cohen et al, Mol. Gen. Genet., 1985, vol. 200, pp. 74-79, "Isolation of the Catalase A Gene of Saccharomyces cerevisiae, by Complementation of the cta1 Mutation".
Longridge et al, EMBOJ, vol. 3(11), pp. 2467-2471, 1927, "Transcription from Maize Storage Protein Gene Promoters in Yeast".
Spevak et al, Mol. Gen. Genet., 1986, vol. 203, pp. 73-78, "Heme Control Region of the Catalase T Gene of the Yeast Saccharomyces cerevisiae".
Obstergaard Jensen et al, EMBOJ, vol. 5 (No. 5), pp. 843-847, 1986, "Heme Regulates the Expression in Saccharomyces cerevisiae, of . . . ".
Hyldig-Nielsen et al, Nucl. Acids Res., vol. 10 (2) 1982, pp. 689-700, "The Primary Structures of Two Leghemoglobin Genes from Soybean".

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