Positive selection vector for the bacteriophage P1 cloning syste

Details Positive selection vector for the bacteriophage P1 cloning syste Positive selection vector for the bacteriophage P1 cloning syste

1991-02-26

1994-04-05

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, 43525233, 935 79, 935 83, 935 84, 536 232, 536 241, C12N 1500, C12N 121, C12N 1570, C12N 1511

Patent

active

053004310

ABSTRACT:
Positive selection cassettes are disclosed which contain a lethal gene, a promoter, a repressor sequence overlapping the promoter, and a cloning site between the promoter and the lethal gene. Insertion of a foreign nucleic acid sequence into the cloning site prevents expression of the lethal gene. Expression of the lethal gene under nonrepressed conditions kills a host organism containing a positive selection cassette which does not contain the foreign nucleic acid sequence.

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