Positive selection vector based on the caps gene, pCAPs vector a

Details Positive selection vector based on the caps gene, pCAPs vector a Positive selection vector based on the caps gene, pCAPs vector a

1997-07-14

1999-04-06

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 9141, 4352523, 43525233, 4353201, 536 231, 536 241, 536 242, C12N 1500, C12P 1934

Patent

active

058916873

ABSTRACT:
A vector according to the invention with a positive selection possibility for cloning a DNA sequence into a host cell transformed with the vector, wherein the vector contains suitable restriction cleavage sites within a selection gene the functional expression of which is prevented by insertion of the foreign gene is characterized in that it contains a modified cap gene as the selection gene which codes for a CAP protein with modified DNA binding specificity the expression of which is lethal for adenylate-cyclase-positive host cells.

REFERENCES:
Ebright et al., Mutation that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli, Nature v. 311, 232 (Sep. 26, 1984).
Henrich et al., Positive-selection vector with enhanced lytic potential based on a variant of phi X174 phage gene E, Gene v. 154, 51-54 ( Mar. 2, 1995).

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