Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-07-04
2006-07-04
Sisson, Bradley L. (Department: 1634)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S091100, C435S187000, C536S023100, C536S024330, C536S025300
Reexamination Certificate
active
07070962
ABSTRACT:
The present invention relates to an improvement in a method for amplifying a target sequence of a target polynucleotide. The method comprises the step of forming extension products of an oligonucleotide primer at least along the target sequence or along an extended oligonucleotide primer. The extension products are copies of the target sequence. The improvement comprises forming the extension products in the presence of a second polynucleotide, to which the oligonucleotide primer hybridizes except for 1–10 nucleotides at the 3′-end of the oligonucleotide primer. Under the conditions chosen, the oligonucleotide primer is extended along the second polynucleotide in a controlled manner relative to extension of such primer along the target sequence, thus providing a positive control for the amplification reaction, which control may be qualitative or quantitative. Optionally, a modified oligonucleotide primer is included in the amplification reaction. The modified primer is substantially identical to the oligonucleotide primer with the exception of a chemical modification at its 3′-end that prevents degradation, under the reaction conditions, of the 1–10 nucleotides referred to above. The method finds particular application in the area of nucleic acid amplification.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5391480 (1995-02-01), Davis et al.
patent: 5427932 (1995-06-01), Weier et al.
patent: 5436149 (1995-07-01), Barnes
patent: 5491086 (1996-02-01), Gelfand et al.
patent: 5491133 (1996-02-01), Walder et al.
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5618664 (1997-04-01), Kiessling
patent: 5627054 (1997-05-01), Gillespie
patent: 6482590 (2002-11-01), Ullman et al.
patent: WO 93/22456 (1993-11-01), None
patent: 94/04706 (1994-03-01), None
patent: 96/41000 (1996-12-01), None
Sommer and Tautz, Minimal homology requirements for PCR primers, Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6749.
S. Diviacco, et al. “A Novel Procedure For Quantitative Polymerase Chain Reaction By Complification Of Competitive Templates”, GENE, vol. 122, No. 2, Jan. 1, 1992, pp. 313-320.
A.M. Wang, et al. “Quantitation Of mRNA By The Polymerase Chain Reaction”, Proceedings Of The National Academy Of Sciences Of USA, vol. 86, No. 24, Dec. 1, 1989, pp. 9717-9721.
A. Skerra, “Phosphorothioate Primers Improve The Amplification Of DNA Sequences By DNA Polymerases With Proofreading Activity”, Nucleic Acids Research, vol. 20, Jul. 25, 1992, No. 14, pp. 3551-3554.
Int'l Search Report dated Aug. 8, 1997 re Int'l Appln. No. PCT/IB97/00226.
Aventis Pharmaceuticals Inc.
Coppola William C.
Sisson Bradley L.
LandOfFree
Positive controls in polynucleotide amplification does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Positive controls in polynucleotide amplification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Positive controls in polynucleotide amplification will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3576437