Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides
Reexamination Certificate
2006-04-18
2006-04-18
Whisenant, Ethan (Department: 1634)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Synthesis of peptides
C530S412000, C435S006120, C536S023100, C536S024300
Reexamination Certificate
active
07030215
ABSTRACT:
The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.
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Jamieson Andrew C.
Liu Qiang
Rebar Edward
Brennan Sean M.
Robins & Pasternak LLP
Sangamo Biosciences, Inc.
Whisenant Ethan
LandOfFree
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