Position-addressable polynucleotide arrays

Details Position-addressable polynucleotide arrays Position-addressable polynucleotide arrays

1995-08-29

1998-03-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 5, 435 912, 536 243, 536 2433, 536 2432, C12P 1934, C12Q 168, C12Q 170, C07H 2104

Patent

active

057233209

ABSTRACT:
A method for producing a high-density, position-addressable gene array is disclosed. The method includes contacting an array of different-sequence oligonucleotides having a unique, known combinatorial sequence associated with each position in the array with a set of extended gene probe templates which are complementary to the oligonucleotides at one of the template end regions. After hybridization, the oligonucleotides in the array are extended by strand-directed polymerization to form the probe array. Also disclosed is a probe array device formed by the method.

REFERENCES:
Fodor et al. Science 251:767-773, Feb. 15, 1991.

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