Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-12-24
1987-12-08
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435253, 4353171, 435 91, 536 27, 935 11, 935 29, 935 41, 935 43, 935 45, 935 73, C12P 2100, C12P 1934, C12N 1500, C12N 120, C12N 100, C07H 1700
Patent
active
047118458
ABSTRACT:
Two types of convenient portable control cassettes for the expression of protein encoding sequences in procaryotes are disclosed. Both cassettes comprise the P.sub.L promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (N.sub.RBS). In one embodiment, this cassette is bordered by restriction sites upstream of the P.sub.L promoter and immediately downstream from the N.sub.RBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.
REFERENCES:
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Rosenberg, M. et al. in Methods in Enzymology, vol. 101, Part C, pp. 123-138, 1983.
Wong, Em. Proc. Natl'. Acad Sci., vol. 79, pp. 3570-3574, 1982.
Shimatake, H. et al., Nature, vol. 292, pp. 128-132, 1981.
Remaut, E. et al., Nuc Acids Res., vol. 11, pp. 4677-4688, 1983.
Remaut, E. et al., Gene, vol. 22, pp. 103-113, 1983.
Taniguchi, T. et al., Nature, vol. 302, pp. 305-310, 1983.
Goeddel, D. V., et al., Nucl Acids Res (1980) 8:4057-4074.
Scherer, G. F. E., et al., Nucl Acids Res (1980) 8:3895-3907.
Gelfand David H.
Lawyer Frances C.
Cetus Corporation
Halluin Albert P.
Huleatt Jayme A.
Murashige Kate H.
Wiseman Thomas G.
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