Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1991-09-30
1994-08-02
Stoll, Robert L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C12Q 146
Patent
active
H00013447
ABSTRACT:
A method is provided for the integration of a low-volume liquid flow and ing system and a portable optical waveguide-based fluorescence detector for the chemical analysis of reagents in fluorescence-based reactions. The method is particularly applicable to the detection of enzyme inhibitors, modifiers, or ligands in cases where a fluorescent enzyme substrate or product exists. In a preferred embodiment, the presence and concentration of acetylcholinesterase inhibitors, such as chemical warfare nerve agents or certain insecticides, is determined by mixing aqueous samples with a dilute solution of n-methyl indoxyl acetate, and monitoring the formation of a fluorescent product (n-methyl indoxyl). The presence of an acetylcholinesterase inhibitor reduces the amount of fluorescent product formed in a given time period. The reaction product passes through a flow cell in which it is excited by the evanescent wave produced by light as the light undergoes total internal reflection within an optical rod or fiber, or by light coming out the end-face of an optical fiber or rod.
REFERENCES:
patent: 4071020 (1978-01-01), Pugliese
patent: 4447546 (1984-05-01), Hirschfeld
patent: 4861727 (1989-08-01), Hauenstein et al.
Baldauf Frederick C.
Busey Brent R.
Thames Kenneth E.
Anthony Joseph D.
Goldberg Edward
Lane Anthony T.
Sachs Michael
Stoll Robert L.
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