Porphyromonas gingivalis arginine-specific proteinase

Details Porphyromonas gingivalis arginine-specific proteinase Porphyromonas gingivalis arginine-specific proteinase

1994-11-08

2000-01-25

Achutamurthy, Ponnathapu

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435220, A61K 3846, C12N 952

Patent

active

06017532&

ABSTRACT:
Provided herein is a Porphyromonas gingivalis high molecular weight arginine-specific proteinase comprising a protease component of 50 kD and a hemagglutinin component of about 44 kD as estimated by SDS-PAGE. The proteinase is stimulated by glycine containing peptides and glycine analogues. It is inhibited by cysteine protease group specific inhibitors.

REFERENCES:
patent: 5475097 (1995-12-01), Travis et al.
Uitto V.J. (1987) "Human Gingival Proteases. 1: Extraction and Preliminary Characterization of Trypsin-like and Elastase-like Enzymes"; J. Periodontal Res. 22:58-63.
Ono et al. (1987) "Purification and Characterization of a Thiol-protease from Bacteroides gingivalis Strain 381"; Oral Micr. Immun. 2:77-81.
Sorsa et al. (1987) "A Trypsin-like Protease from Bacteroides gingivalis: Partial Purification and Characterization"; J. Periodont Res. 22:375-380.
Otsuka et al. (1987) "Isolation and Characterization of Protease from Culture Supernatant of Bacteroides gingivalis"; J. Periodont Res. 22:491-498.
Fujimura et al. (1987) "Isolation and Characterization of a Protease from Bacteroides gingivalis" Infect. Immun. 3(vol.55):716-720.
Otogoto et al. (1993) "Isolation and Characterization of the Porphyromonas gingivalis prT Gene, Coding for Protease Activity", Infect. & Immun. 1(61):117-123.
Park et al. (1992) "Cloning of a Porphyromonas (Bacteroides) gingivalis Protease Gene and Characterization of its Product", FEMS Microb. Lett. 92:273-278.
Shah et al. (1992) "Evidence for Independent Molecular Identity and Functional Interaction of the Haemagglutinin and Cysteine Proteinase (gingivain) of Porphyromonas gingivalis", J.Med. Microbiol. 36:239-244.
Nishikata et al. (1991) "Characterization of Porphyromonas (Bacteroides) gingivalis Hemagglutinin as as Protease", Biochem. & Biophys. Res. Comm. 1(178):336-342.
Roberts et al. (1990) "Purification of the Secreted Thiol-activated Protease of Porphyromonas (Bacteroides) gingivalis and the Cloning and Expression of the Gene in Escherichia coli", Clinical & Molecular Aspects of Anaerobes (ed.) S.P. Borriello, pp. 227-233.
Lee et al. (1988), "Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase", Science 239:1288-1291.
Chen et al. (1992) "Purification and Characterization of a 50-kDa Cysteine Proteinase (Gingipain) from Porphyromonas gingivalis," J. Biol. Chem. 267: 18896-18901.
Wingrove et al. ((1992) "Activation of Complement Components C3 and C5 by a Cysteine Proteinase (Gingipain-1) from Porphyromonas (Bacteroides) gingivalis," J. Biol. Chem. 287: 18902-18907.
Chen et al. (1991) "Stimulation of Proteinase and Amidase Activities in Porphyromonas (Bacteroides) gingivalis by Amino Acids and Dipeptides," Infect. Immun. 59: 2846-2850.
Travis et al. (1991) "Bacterial Proteinases in Periodontal Disease," Abstract, J. Cell Biochem. Suppl.O (15 Part G), 117.
Potempa et al. (1991) "Purification and Characterization of a 50 kD Cysteine Proteinase of Porphyromonas gingivalis," Abstract, FASEB J. 5(4) A829.
Hugli et al. (1991) "A Role for Complement in Gingivitis: Acitivation by a Cysteine Protease from Porphyromonas gingivalis," Abstract, Clin. Exp. Immunol. 86(Suppl.1), 20.
Schenkein, H.A. (1988) "The Effect of Periodontal Proteolytic Bacteroides Species on Proteins of the Human Complement System," J. Periodontal Res. 23: 187-192.
Tsutsui et al. (1987) "Purification and Characterization of a Protease from Bacteroides gingivalis 381," Infect. Immun. 55: 420-427.
Yoshimura et al. (1984) "Characterization of a Trypsin-Like Protease from the Bacterium Bacteroides gingivalis Isolated from Human Dental Plaque," Archs Oral Biol. 29: 559-564.
Suido et al. (1987) "Characterization of N-CBz-glycyl-glycyl-arginyl peptidase and glycyl-prolyl peptidase of Bacteroides gingivalis," J. Periodont. Res. 22: 412-418.
Birkedal-Hansen et al. (1988) "Characterization of Collagenolytic Activity from Strains of Bacteroides gingivalis," J. Periodontal Res. 23: 258-264.
Grenier et al. (1989) "Characterization of Sodium Dodecyl Sulfate-Stable Bacteroides gingivalis Proteases by Polyacrylamide Gel Electrophoresis," Infect. Immun. 57: 95-99.

Affiliated with

Also associated with

Rating

Porphyromonas gingivalis arginine-specific proteinase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Porphyromonas gingivalis arginine-specific proteinase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Porphyromonas gingivalis arginine-specific proteinase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2313851