Pop up electrophoresis apparatus and method

Details Pop up electrophoresis apparatus and method Pop up electrophoresis apparatus and method

1988-07-15

1989-12-26

Niebling, John F.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, G01N 2728, G01N 2726

Patent

active

048896101

ABSTRACT:
An apparatus provides for casting a gel and for presenting the gel for electrophoresis. The apparatus comprises a casting tray and the casting plate which combine to form a mold for the gelable liquid, and an electrophoresis tank in which the electrophoresis process occurs. The floor of the electrophoresis tank rises to form the plate pedestal upon which the casting plate rests during electrophoresis. The floor of the electrophoresis tank forms a cavity at the edges of the plate pedestal in which the casting tray rests during the electrophoresis process. The casting plate is first firmly seated in the bottom of the casting tray forming a mold for the gelable liquid. The gelable liquid is poured into the mold. The comb is placed in the comb slots so as to form wells in the gel. The gelable liquid is allowed to gel, and the comb is removed. The material to undergo electrophoresis is placed in the wells left by the comb. The entire assembly is then placed and aligned on the plate pedestal. The casting tray is then pushed down into the casting tray well. Electrophoresis buffer sufficient to fill the tank to approximately the top of the gel is then poured into the electrophoresis tank. An alternate embodiment is shown.

REFERENCES:
patent: 3932265 (1976-01-01), Hoefer
patent: 4035377 (1977-07-01), Detroy
patent: 4049534 (1977-09-01), Posner
patent: 4151065 (1979-04-01), Kaplan et al.
patent: 4234400 (1980-11-01), Kaplan et al.
patent: 4292161 (1981-09-01), Hoefer et al.
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4574040 (1986-03-01), Delony et al.
patent: 4588491 (1986-05-01), Kreisher et al.
patent: 4618408 (1986-10-01), Malavarca et al.
patent: 4624768 (1986-11-01), Yoshida et al.
patent: 4657655 (1987-04-01), Smoot et al.
patent: 4693804 (1987-09-01), Serwer
Advertisement entitled "Astec Band-It" (Mechanical Drawing Dated Mar., 1987, Citing U.S. Pat. No. 4,618,408 to Malavarca et al., issued 10/1986.
"Analysis of Restriction Fragments of T7 DNA and Determination of Molecular Weights by Electrophoresis in Neutral and Alkaline Gels," McDonell et al., J. Md. Biol. (1977), 119-146.
"Separation of Chromosome-Sized DNA Molecules by Pulsed Field Gel Electrophoresis," Lex H. T. Van Der Ploeg, (Jan./Feb. 1987).

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