Polysilicon binding assay support and methods

Details Polysilicon binding assay support and methods Polysilicon binding assay support and methods

1987-03-26

1989-12-12

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436501, 436524, 436810, 422 55, 422 57, 422 58, G01N 33543, G01N 33566, G01N 2100, G01N 2106

Patent

active

048867616

ABSTRACT:
An improved binding assay plate having greatly reduced non-specific binding with proteinaceous reagents comprising a polysilicon surface having a binding reagent adhered thereto, the surface having a coating adsorbed thereon of a proteinaceous non-specific binding inhibitor. This binding assay product is prepared by adhering binding reagent to a polysilicon surface and then treating the surface with the proteinaceous non-specific binding inhibitor.
The binding assay method comprises the steps of contacting the polysilicion surface having a primary binding reagent and non-specific binding inhibitor adhered to the surface thereof with a solution containing an analyte with which the primary binding reagent specifically binds for a time sufficient to permit conjugation of analyte with the primary binding reagent and determining the analyte bound to the polysilicon surface. The plate is suitable for both sandwich and competition immunoassays. The reduction of non-specific binding produces a substantial increase in sensitivity.

REFERENCES:
patent: 4299916 (1981-11-01), Litman et al.
patent: 4334880 (1982-06-01), Malmros
patent: 4525452 (1985-06-01), Jones et al.
patent: 4554088 (1985-11-01), Whitehead et al.

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