Polysaccharide producing process using protease and Azotobacter

Details Polysaccharide producing process using protease and Azotobacter Polysaccharide producing process using protease and Azotobacter

1978-03-10

1980-11-18

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435274, 435813, 435831, C12P 1904, C13L 300, C12R 1065

Patent

active

042346886

ABSTRACT:
The solution viscosity of a polysaccharide, microbial alginate, produced by culturing strains of Azotobacter vinelandii, is controlled by addition of a protease to the culture broth. The protease can be added during the culture, to obtain a polysaccharide of increased viscosity. Alternatively or additionally protease can also be added after culture but before the polysaccharide is isolated from the broth, for example, to prevent reduction of viscosity during storage. Neutral and alkaline proteases having activity at around pH 7 are particularly suitable.

REFERENCES:
patent: 3856625 (1974-12-01), Imrie
patent: 3966618 (1976-06-01), Colegrove
patent: 4094739 (1978-06-01), Schroeck
patent: 4130461 (1978-12-01), Righelato et al.
Larsen, et al., "BiosynThesis of Alginate", Carbohyd. Res. vol. 17, (1971), pp. 287-308.

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