Polysaccharide polymer made by xanthomonas

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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514 54, 536114, 435104, 252 8551, C08B 3700, C08B 3718

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active

047134496

ABSTRACT:
A polysaccharide polymer is disclosed which is a better viscosifier of water than xanthan gum. The polysaccharide polymer and its non-acetylated form, are comprised of glucose and mannose moieties in a ratio of about 2:1. The invention also discloses Xanthomonas mutants which produce the polysaccharide polymer but which do not produce xanthan gum. Methods of preparing the polysaccharide polymers and of their use are also described.

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Ielpi, L., Couso, R., and Dankert, M., "Lipid Linked Intermediates in the Biosynthesis of Xanthan Gum," FEBS Letters, 130:253-256, (1981).
Ielpi, L., Couso, R., and Dankert, M. A., "Xanthan Gum Biosynthesis Pyruvic Acid Acetal Residues are Transferred from Phosphoenol Pyruvate to the Pentasaccharide-P-PLipid." Biochem. Biophys. Res. Comm., 102:1400-1408, (1981).
Ielpi, L., Couso, R., and Dankert, M. A., "Xanthan Gum Biosynthesis Acetylation Occurs at the Prenyl-Phospho-Sugan Stage," Biochem. Intern., 6:323-333, (1983).
Osborn, M. J. and Weiner, I. M., "Biosynthesis of a Bacterial Lipopolysaccharide." J. Biol. Chem., 243:2631-2639, (1967).
Colvin, J. R., Chene, L., Sowden, L. C., and Takai, M., "Purification and Properties of a Soluble Polymer of Glucose from Cultures of Acetobacter zylinum," Can. J. Biochem., 55:1057-1063, (1977).
Johnson, J. G. and Wilson, D. B., "Role of a Sugar-Lipid Intermediate in Colanic Acid Synthesis by Escherichia coli," J. Bact., 129:225-236, (1977).

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