Polypeptides having phospholipase A 2 activity

Details Polypeptides having phospholipase A 2 activity Polypeptides having phospholipase A 2 activity

2007-08-21

2007-08-21

Prouty, Rebecca E. (Department: 1652)

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

C435S196000, C435S320100, C435S018000, C435S252300, C435S069100, C435S006120, C435S094000, C536S023200

Reexamination Certificate

active

10380873

ABSTRACT:
The present invention relates to a novel phospholipase A2polypeptide, DNA encoding the polypeptide, a vector comprising the DNA, a transformant transformed with the vector, and a process for producing the phospholipase A2polypeptide. The present invention also relates to a method of utilizing the polypeptide, e.g., a method of screening for a compound having agonist or antagonist activity by using the polypeptide or an antibody to the polypeptide, and a pharmaceutical comprising the polypeptide or an antibody to the polypeptide. The present invention further relates to a polypeptide inhibiting the phospholipase A2activity of a phospholipase A2polypeptide (hereinafter referred to as inhibitor polypeptide), DNA encoding the inhibitor polypeptide, a vector comprising the DNA encoding the inhibitor polypeptide, a transformant transformed with the vector, a pharmaceutical comprising the inhibitor polypeptide, and a process for producing the inhibitor polypeptide.

REFERENCES:
patent: 6025178 (2000-02-01), Choiu et al.
patent: 00/24911 (2000-05-01), None
patent: 00/47763 (2000-08-01), None
patent: WO/00/47763 (2000-08-01), None
patent: 01/53326 (2001-07-01), None
patent: 01/53455 (2001-07-01), None
patent: WO 01/53326 (2001-07-01), None
patent: 01/54477 (2001-08-01), None
patent: 01/92523 (2001-12-01), None
patent: 02/04490 (2002-01-01), None
patent: 02/31125 (2002-04-01), None
Perisic et al. Mapping the phospholipid-binding surface and translocation determinants of the C2 domain from cytosolic phospholipase A2. J Biol Chem. May 21, 1999;274(21):14979-87.
Pickard, et al., “Molecular Cloning of Two New Human Paralogs of 85-kDa Cytosolic Phospholipase A2”,The Journal of Biological Chemistry, vol. 274, No. 13 (1999), pp. 8823-8831.
Database Genebank, “Homo sapienscytosolic phospholipase A2 beta (cPLA2 beta) mRNA, complete cds”, (XP002306372) Database Accession No. AF065215 (1999).
Pickard, et al., “Identification of Essential Residues for the Catalytic Function of 85-kDa Cytosolic Phospholipase A2”,The Journal of Biological Chemistry, vol. 271, No. 32 (1996), pp. 19225-19231.
Tay, et al., “Isolation of promoter for cytosolic phospholipase A2(cPLA2)”,Biochimica et Biophysica Acta, vol. 1217, No. 3 (1994), pp. 345-347.
Sharp, et al., “Molecular Cloning and Expression of Human Ca2+-sensitive Cytosolic Phospholipase A2”,The Journal of Biological Chemistry, vol. 266, No. 23 (1991), pp. 14850-14853.
Database Genebank, Phosphatidylcholine 2-acylhydrolase ‘Homosapiens’ (1999), (XP-002306373), Database Accession No. AAA60105.
Sharp, et al. “Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2”,The Journal of Biological Chemistry, vol. 269, No. 37 (1994), pp. 23250-23254.
Chiba, et al., “Cloning of a Gene for a Novel Epitheium-specific Cytosolic Phospholipase A2, cPLA2δ, Induced in Psoriatic Skin”,The Journal of Biological Chemistry, vol. 279, No. 13 (2004), pp. 12890-12897.
Kramer, et al., “Structure, function and regulation of Ca2+-sensitive cytosolic phospholipase A2 (cPLA2)”,FEBS Letters, vol. 410, No. 1 (1997), pp. 49-53.
Leslie, “Properties and Regulation of Cytosolic Phospholipase A2”,The Journal of Biological Chemistry, vol. 272, No. 27 (1997), pp. 16709-16712.
Exton, “Phosphatidylcholine breakdown and signal transduction”,Biochimica et Biophysica Acta, vol. 1212, No. 1 (1994), pp. 26-42.
Hirabayashi, et al., “Regulatory Mechanism and Physiological Role of Cytosolic Phospholipase A2”,Biol. Phar. Bull, vol. 27, No. 8 (2004), pp. 1168-1173.
Ramanadham, et al., “Type IB secretory phospholipase A2is contained in insulin secretory granules of . . . ”,Biochimica et Biophysica Acta, vol. 1390, No. 3 (1998), pp. 301-312.

Affiliated with

Also associated with

Rating

Polypeptides having phospholipase A 2 activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polypeptides having phospholipase A 2 activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptides having phospholipase A 2 activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3840275