Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Reexamination Certificate
2000-10-31
2002-08-13
Saidha, Tekchand (Department: 1652)
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
C435S196000, C435S252300, C435S320100, C435S929000, C536S023200
Reexamination Certificate
active
06432898
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to isolated polypeptides having lipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
2. Description of the Related Art
Lipases (EC 3.1.1.3) are enzymes that can hydrolyze triglycerides to release fatty acid.
Detergents formulated with lipolytic enzymes are known to have improved properties for removing fatty stains. For example, LIPOLASE™ (Novo Nordisk A/S, Bagsvaerd, Denmark), a microbial lipase obtained from the fungus
Thermomyces lanuginosus
(also called
Humicola lanuginosa
), has been introduced into many commercial brands of detergent.
WO 98/26057 discloses a polypeptide having lipase and phospholipase activity (GenBank Acc. No. A85215) obtained from
Fusarium oxysporum.
It is an object of the present invention to provide improved polypeptides having lipase activity and nucleic acid encoding the polypeptides.
SUMMARY OF THE INVENTION
The present invention relates to isolated polypeptides having lipase activity selected from the group consisting of:
(a) a polypeptide having an amino acid sequence which has at least 85% identity with amino acids 31 to 349 of SEQ ID NO:2;
(b) a polypeptide encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 1525 to 2530 of SEQ ID NO:1, (ii) the cDNA sequence contained in nucleotides 1525 to 2530 of SEQ ID NO:1, (iii) a subsequence of (i) or (ii) of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii), or (iii);
(c) a variant of the polypeptide having an amino acid sequence of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion of one or more amino acids; and
(d) a fragment of (a) or (b) that has lipase activity.
The present invention also relates to isolated nucleic acid sequences encoding the polypeptides and to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
REFERENCES:
patent: 0869167 (1997-12-01), None
patent: WO 98/26057 (1998-06-01), None
Sequence alignment of SEQ ID No : 1 & 2 With accession Nos. V07424 and W51767 disclosed in WO 98/26057.
Golightly Elizabeth J.
Rey Michael W.
Novozymes Biotech Inc.
Saidha Tekchand
Starnes Robert L.
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