Polypeptides having brain-localizing activity and uses thereof

Details Polypeptides having brain-localizing activity and uses thereof Polypeptides having brain-localizing activity and uses thereof

2011-04-19

2011-04-19

Kemmerer, Elizabeth C. (Department: 1649)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C530S300000, C530S317000, C530S333000, C530S328000, C514S001200, C514S017700, C424S009100, C424S093600, C424S417000, C424S418000, C424S450000, C435S069100

Reexamination Certificate

active

07927811

ABSTRACT:
The present invention relates to polypeptides having a brain-localizing activity, molecules comprising these polypeptides, and pharmaceutical agents that confer brain-localizing activity. The present inventors are the first to reveal amino acid motif sequences involved in brain-localizing activity. Polypeptides that comprise such motif sequences and have brain localizing activity were discovered as follows: DNAs encoding polypeptides comprising random amino acid sequences were synthesized, and incorporated into a phage library. The phage library produced was used to screen for polypeptides having brain-localized activity, which yielded such several polypeptides. These polypeptides comprised common sequences, which lead to the successful discovery of amino acid motif sequences involved in brain-localizing activity. The polypeptides of the present invention comprising the motif sequences specifically bind to cerebrovascular endothelial cells, and induce a transcellular pathway that enables brain-specific targeting and transport of substances in the cerebral parenchyma, which was not possible prior to the present invention.

REFERENCES:
patent: 5622699 (1997-04-01), Ruoslahti et al.
patent: 6007996 (1999-12-01), McNamara et al.
patent: 6274552 (2001-08-01), Tamarkin et al.
patent: 6562958 (2003-05-01), Breton et al.
patent: 7214786 (2007-05-01), Kovalic et al.
patent: 2007/0254316 (2007-11-01), Rodriguez et al.
patent: 2007/0269523 (2007-11-01), Sawada et al.
patent: 2009/0092583 (2009-04-01), Sawada et al.
patent: 2010/0111838 (2010-05-01), Nakajo et al.
patent: 8-511236 (1996-11-01), None
patent: 11-92406 (1999-04-01), None
patent: 2004-073964 (2004-03-01), None
patent: WO 02/087509 (2002-11-01), None
patent: WO 03/009881 (2003-02-01), None
patent: WO 03/020751 (2003-03-01), None
patent: WO 2004/015392 (2004-02-01), None
Muruganandam et al., “Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood-brain barrier endothelium,” FASEB J., Feb. 2002, vol. 16, No. 2, pp. 240-242.
Shi et al., “Noninvasive gene targeting to the brain,” Proc. Natl. Acad. Sci. USA, Jun. 2000, vol. 97, No. 13, pp. 7567-7572.
Imai et al., “Migration activity of microglia and macrophages into rat brain,” Neuroscience Letters, 1997, 237:49-52.
Kang et al., “Stability of the Disulfide Bond in an Avidin-Biotin Linked Chimeric Peptide During in vivo Transcytosis Through Brain Endothelial Cells”, Journal of Drug Targeting, 2000, vol. 8, No. 6, pp. 425-434.
Pasqualini et al., “Organ targeting in vivo using phage display in vivo using phage display peptide libraries”, Nature, 1996, vol. 380, No. 6572, pp. 364-366.
Sawada et al., “Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain”, FEBS Letters, 1998, vol. 433, pp. 37-40.
Schwarze, et al., “In vivo protein transduction: delivery of a biologically active protein into the mouse”, Science, 1999, vol. 285, No. 5433, pp. 1569-1572.
R. Pasqualini et al., “Organ targeting in vivo using phage display in vivo using phage display peptide libraries”, Nature, 1996, vol. 380, No. 6572, pp. 364-366.
S.R. Schwarze, et al., “In vivo protein transduction: delivery of a biologically active protein into the mouse”, Science, 1999, vol. 285, No. 5433, pp. 1569-1572.
Young-Sook Kang et al., “Stability of the Disulfide Bond in an Avidin-Biotin Linked Chimeric Peptide During in vivo Transcytosis Through Brain Endothelial Cells”, Journal of Drug Targeting, 2000, vol. 8, No. 6, pp. 425-434.
Makoto Sawada et al., “Brain-specific gene expression by immortalized microglial cell-mediated gene transfer in the mammalian brain”, FEBS Letters, 1998, vol. 433, pp. 37-40.
Egleton et al., “Transport of Opioid Peptides into the Central Nervous System,” Journal of Pharmaceutical Sciences, Nov. 1998, 87(11):1433-1439.
Piwko et al., “Localization and Pharmacological Characterization of Somatostatin Recognition Sites in the Human Cerebellum,” Neuropharmacology, Jun. 1996, 35(6):713-723.
Supplementary European Search Report mailed Apr. 8, 2009, in European Patent Application EP 06783020), 4 pages.
Boder et al., “Yeast surface display for screening combinatorial polypeptide libraries,” Nature Biotechnology, Jun. 1, 1997, 15:553-557.
Kim et al., “Human neural stem cells genetically modified for brain repair in neurological disorders,” Neuropathology: Official Journal of the Japanese Society of Neuropathology, Sep. 2004, 24(3):159-171.
Kondo et al., “Yeast cell-surface display-applications of molecular display,” Applied Microbiology and Biotechnology, Mar. 1, 2004, 64(1):28-40.
Laakkonen et al., “A tumor-homing peptide with a targeting specificity related to lymphatic vessels,” Nature Medicine, Jun. 10, 2002, 8(7):751-755.
Laakkonen et al., “Antitumor activity of a homing peptide that targets tumor lymphatics and tumor cells,” Proc. Natl. Acad. Sci., Jun. 22, 2004, 101(25):9381-9386.
Makar et al., “Brain-derived neurotrophic factor (BDNF) gene delivery into the CNS using bone marrow cells as vehicles in mice,” Neuroscience Letters, Feb. 17, 2004, 356:215-219.
Bickel et al., “In Vivo Demonstration of Subcellular Localization of Anti-transferrin Receptor Monoclonal Antibody-Colloidal Gold Conjugate in Brain Capillary Endothelium,” The Journal of Histochemistry and Cytochemistry, 1994, 42(11):1493-1497.

Affiliated with

Also associated with

Rating

Polypeptides having brain-localizing activity and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Polypeptides having brain-localizing activity and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptides having brain-localizing activity and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2709174