Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
1999-02-25
2002-09-10
Park, Hankyel T. (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C424S192100, C424S196110, C424S199100, C424S224100, C424S208100, C435S069510, C435S414000, C435S419000, C800S278000, C800S280000, C800S288000
Reexamination Certificate
active
06448070
ABSTRACT:
FIELD OF THE INVENTION
The field of the invention is recombinant plant viruses, especially their use as immunizing agents which carry antigenic sequences from mammalian (e.g., human) or other animal pathogens and their use as a system for increased production of polypeptides of interest.
BACKGROUND OF THE INVENTION
Traditionally, successful vaccination has been dependent upon the use of live attenuated viruses or preparations of killed pathogenic organisms. These vaccines are very effective in controlling or, as in the case of smallpox, even eliminating certain infectious diseases. However, their use often present safety concerns. Subunit vaccines based on peptide or proteins derived from a pathogen are less hazardous than traditional vaccines but have generally suffered from poor immunogenicity and high expense. Moreover, current vaccines with a few exceptions must be administered parenterally. However, it is well known that most pathogens gain entry across the mucosal surfaces of the body and a mucosal immune response would therefore be more appropriate.
Both safety concerns and the desire to target mucosal tissues for more effective immunization against common pathogens dictate the need for new approaches to vaccination. For induction of a mucosal response, oral administration of antigen is appropriate, inexpensive, and safe. However, in order to efficiently immunize by the oral route, several obstacles such as degradation from low pH or proteases in the gastrointestinal GI tract, the short exposure to immune induction sites, and limited permeability must be overcome.
Recent studies demonstrate that plants and plant viruses can function as effective tools for vaccine production and delivery. Furthermore, like liposomes and microcapsules, it is expected that plant cells and plant viruses will serve as delivery vehicles providing natural protection for the antigen associated with them and enhancing the uptake of the antigen from the GI tract. Such new developing “green system vaccines” have significant advantages over the traditional and synthetic vaccines as regards safety, deliverability via either parenteral, nasal or oral routes, and lower cost of production.
BRIEF SUMMARY OF THE INVENTION
In one general aspect, the current invention is a process of delivering a fusion capsid protein (a plant virus capsid protein fused to a foreign polypeptide) to a mammal (such as a human) or other animal using recombinant tobacco mosaic virus (TMV) genetic material (TMV genetic material combined with genetic material that codes for a chimeric capsid protein, the chimeric capsid protein being capsid protein of either an alfalfa mosaic virus (AlMV) capsid protein (CP) or ilarvirus CP, fused to the foreign polypeptide) as a delivery vehicle to that mammal or other animal. A foreign polypeptide is one that does not naturally occur in either TMV, an AlMV or an ilarvirus. The fusion protein is administered to the mammal or other animal for purposes of inducing an immune response against the foreign polypeptide. In a second general aspect, the invention is a production process: the use of such a chimeric virus to express the fused coat protein (comprising either an antigenic or nonantigenic foreign protein) in a plant.
REFERENCES:
patent: 4956282 (1990-09-01), Goodman et al.
patent: 5041385 (1991-08-01), Kingsman et al.
patent: 5316931 (1994-05-01), Donson et al.
patent: 5466788 (1995-11-01), Ahlquist
patent: 5500360 (1996-03-01), Ahlquist
patent: 5589367 (1996-12-01), Donson et al.
patent: 5612487 (1997-03-01), Lam et al.
patent: 5862541 (1999-01-01), Samulski
patent: 0174759 (1991-04-01), None
patent: WO9218618 (1992-10-01), None
patent: WO-96/120128 (1996-04-01), None
C. Porta et al., Development of Cowpea Mosaic Virus as a High-Yielding System for the Presentation of Foreign Peptides,Virologyvol. 202, 949-955 (1994).
T.H. Turpen et al., Malarial Epitopes Expressed on the Surface of Recombinant Tobacco Mosaic Virus;Bio/Technology, vol. 13, 53-57 (1995).
L. McLain et al., Human Immunodeficiency Virus Type 1-Neutralizing Antibodies Raised to a Glycoprotein 41 Peptide Expressed on the Surface of a Plant Virus;Aids Res. and Human Retroviruses, vol. 11, 327-334, (1995).
J. Fitchen et al. Plant Virus Expressing Hybrid Coat Protein With Added Murine Epitope Elicits Autoantibody Response;Vaccine, vol. 13, 1051-1057 (1995).
R. Usha et al., Expression of an Animal Virus Antigenic Site on the Surface Of a Plant Virus Particle;Virology, vol. 197, 366-374 (1993).
Vidadi Yusibov and Sue Loesch-Fries, High Affinity RNA-Binding Domains of Alfalfa Mosaic Virus Coat Protein are Not Required for Coat Protein-Mediated Resistance;Proc. National Academy of Science.U.S., vol. 92, 8980-8984 (1995).
Vidadi Yusibov, et al., Purification, Characterization, Assembly and Crystallization Of Assembled Alfalfa Mosaic Virus Coat Protein Expressed inEscherichia coli,J. Gen. Virol, vol. 77, 567-573 (1996).
Bear et al., EMBO J., vol. 13, No. 3, 1994, pp. 727-735.
Donson et al., PNAS USA, vol. 88, Aug. 1991, pp. 7204-7208.
Hooper Douglas Craig
Koprowski Hilary
Modelska Anna
Yusibov Vidadi
Brown Stacy S.
Kumar Nanda P. B. A.
McNichol, Jr. William J.
ReedSmith LLP
Thomas Jefferson University
LandOfFree
Polypeptides fused with alfalfa mosaic virus or ilarvirus... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Polypeptides fused with alfalfa mosaic virus or ilarvirus..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Polypeptides fused with alfalfa mosaic virus or ilarvirus... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2827494