Polypeptides and polypeptide subunits of a stereospecific nitril

Details Polypeptides and polypeptide subunits of a stereospecific nitril Polypeptides and polypeptide subunits of a stereospecific nitril

1998-06-24

2000-10-17

Slobodyansky, Elizabeth

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435232, 4352533, 435877, 536 237, C07K 1421, C12N 988, C12N 120, C12N 100, C07H 2104

Patent

active

061334219

ABSTRACT:
350 The present invention provides a nitrile hydratase nucleic acid fragment isolated from Pseudomonas putida which encodes a nitrile hydratase activity capable of catalyzing the hydrolysis of certain racemic nitriles to the corresponding R- or S-amides. Also provided are transformed microorganisms capable of the active expression of said nitrile hydratase activity. Additionally, the invention provides a transformant harboring the nitrile hydratase gene in conjunction with an amidase gene, both of which may be co-expressed producing active nitrile hydratase and amidase enzymes respectively. Methods for the production of such enantiomeric materials are also provided.

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