Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Plant cell or cell line – per se – contains exogenous or...
Patent
1995-10-06
1999-03-09
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Plant cell or cell line, per se, contains exogenous or...
4351723, 435424, 536 236, C12N 510, C12N 1500, C07H 2104
Patent
active
058799419
ABSTRACT:
Amino acid and nucleotide sequences relating to the glutamate dehydrogenase (GDH) enzyme are described. The GDH enzymes described herein were discovered in the alga Chlorella sorokiniana in the form of seven different inducible isoenzymes. These isoenzymes are found in the algae as chloroplast-localized hexamers composed of .alpha.- and .beta.-subunits. Plants transformed with nucleotide sequences encoding the .alpha.- or .beta.-subunits of the enzyme show improved properties, for example, increased growth and improved stress tolerance.
REFERENCES:
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"Nicotinamide Adenine DI Nucleotide Glutamate Dejydrogenase Obtain Chlorella Cell Buffer Extract Two Stage Chromatography Phosphate Buffer Elution" (1982) Biochem. Inst., **abstract only.
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Bascomb, N.F., R.R. Schmidt (1987) "Purification and Partial Kinetic and Physical Characterization of Two Chloroplast-Localized NADP-Specific Glutamate Dehydrogenase Isoenzymes and Their Preferential Accumulation of Chlorella sorokiniana Cells Cultures at Low or High Ammonium Levels" Plant Physiol. 83:75-84.
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Prunkard, D.E. et al. (1986) "Evidence for Chloroplastic Localization of an Ammonium-Inducible Glutamate Dehydrogenase and Synthesis of its Subunit from a Cytosolic Precursor-Protein in Chlorella sorokiniana" Plant Physiol. 81:349-355.
Wallagrove, R.M. et al. (1987) "Barley Mutants Lacking Chloroplast Glutamine Synthetase-Biochemical and Genetic Analysis" Plant Physiol. 83:155-158.
Miller, P.W. et al. (1994) "Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-cultured Chlorella sorokiniana cells" Journal of Applied Phycology 6:211-223.
Meredith, M.J., R.R. Schmidt (1991) "NAD-Specific glutamate dehydrogenase isoenzyme localised in mitochondria of nitrate-cultured Chlorella sorokiniana cells" Plant Physiol. 10:67-71.
Bascomb, N.F. et al. (1986) "Specific Polysome Innunoadsorption to Purify an Ammonium-Inducible Glutamate Dehydrogenase mRNA from Chlorella sorokiniana and Synthesis of Full Length Double-Stranded cDNA from the Purified mRNA" Plant Physiol. 81:527-532.
Miflin, B.J., P.J. Lea (1976) "The Pathway of Nitrogen Assimilation in Plants" Phytochemistry 15:873-885.
Miller Philip
Schmidt Robert R.
Robinson Douglas W.
University of Florida
Wai Thanda
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