Polypeptide with cell-spreading activity

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530382, C07K 1300

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active

051029880

ABSTRACT:
A polypeptide having the cell-spreading activity of human fibronectin. Methods of preparing the polypeptide are described.

REFERENCES:
patent: 4517686 (1985-05-01), Ruoslahti et al.
patent: 4589881 (1986-05-01), Pierschbacher et al.
patent: 4614517 (1986-09-01), Ruoslahti et al.
The Journal of Biological Chemistry, "Domain Structure of the Carboxylterminal Half of Human Plasma", vol. 258, No. 5, Issue of Mar. 10, pp. 3332-3340, 1983.
K. Sekiguchi and S. Hakomori, "Functional Domain Structure of fibronectin." Proc. Natl. Acad. Sci, U.S.A., 77, 2661-2665, May 1980.
M. D. Pierschbacher, E. G. Hayman and E. Ruoslahti, "Location of the Cell-Attachment Site in Fibronectin with Monoclonal Antibodies and Proteolytic Fragments of the Molecule." Cell, 26, 259-267, Oct. 1981.
M. D. Pierschbacher and E. Ruoslahti, "The Cell Attachment Domain of Fibronectin. Determination of the Primary Structure." J. Biol. Chem., 257, 9593-9597, Aug. 1982.
M. D. Pierschbacher, E. G. Hayman and E. Ruoslahti, "Synthetic Peptide with Cell Attachment Activity of Fibronectin." Proc. Natl. Acad. Sci. U.S.A., 80, 1224-1227, Mar. 1983.
M. D. Pierschbacher and E. Ruoslahti, "Cell Attachment Activity of Fibronectin Can Be Duplicated by Small Synthetic Fragments of the Molecule." Nature, 309, 30-33, May 1984.
A. R. Kiornblihtt, K. Vibe-Pedersen and F. E. Baralle, "Human Fibronectin: Cell Specific Alternative mRNA Splicing Generates Polypeptide Chains Differing in the Number of Internal Repeats." Nucl. Acids. Res., 12, 5853-5868, Jul. 1984.
M. D. Pierschbacher and E. Ruoslahti, "Variants of the Cell Recognition Site of Fibronectin that Retain Attachment-Promoting Activity." Proc. Natl. Acad. Sci. U.S.A., 81, 5985-5988, Oct. 1984.
A. R. Kornblihtt, K. Umezawa, K. Vibe-Pedersen and F. D. Baralle, "Primary Structure of Human Fibronectin: Differential Splicing May Generate at Least 10 Polypeptides From a Single Gene." EMBO J., 4, 1755-1759, 1985.
S. K. Akiyama, E. Hasegawa, T. Hasegawa and K. M. Yamada, "The Interaction of Fibronectin Fragments with Fibroblastic Cells." J. Biol. Chem., 260, 13256-13260, Oct. 1985.
M. Obara, M. S. Kang, S. Rocher-Dufour, A. Kornblihtt, J. P. Thiery and K. M. Yamada, "Expression of the Cell-Binding Domain of Human Fibronectin in E. coli." FEBS Lett., 213, 261-264, Mar. 1987.
The Journal of Biological Chemistry, "The Interaction of Fibronectin Fragments with Fibroblastic Cells", vol. 260, No. 24, Issue of Oct. 25, pp. 13256-13260, 1985.

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